RAD52 anticorps (AA 360-375)

N° du produit ABIN117934

Détail du produit anti-RAD52 anticorps

Antigène: RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

Épitope: AA 360-375

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RAD52 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Séquence: Human RAD52, 419 aa, predicted MW 46.3 kDa: 1 msgteeailg grdshpaagg gsvlcfgqcq ytaeeyqaiq kalrqrlgpe yissrmaggg 61 qkvcyieghr vinlanemfg yngwahsitq qnvdfvdlnn gkfyvgvcaf vrvqlkdgsy 121 hedvgygvse glkskalsle karkeavtdg lkralrsfgn algncildkd ylrslnklpr 181 qlplevdltk akrqdlepsv eearynscrp nmalghpqlq qvtspsrpsh avipadqdcs 241 srslsssave seathqrklr qkqlqqqfre rmekqqvrvs tpsaekseaa ppappvthst 301 pvtvseplle kdflagvtqe liktlednse kwavtpdagd gvvkpssrad paqtsdtlal 361 nnqmvtqnrt phsvchqkpq aksgswdlqt ysadqrttgn weshrksqdm kkrkydpsy

Specificité: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human RAD52 protein. Cross reactivity with RAD52 protein from non-human sources is not expected based on a lack of homology (BLAST) with other known proteins.

Purification: Immunoaffinity chromatography.

Immunogène: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 360-375 of the Human Rad 52 protein.

Information d'application

Indications d'application: This affinity purified antibody has been tested for use in Western blotting (1: 500-1: 2,000)using HeLa nuclear extracts. The antibody is also functional by ELISA (1: 5,000-1: 20,000). Dilutions for Western blotting represent a starting point dilution and further optimizationmay be required. The antibody detects a band of approximately 63.0 kDa (predictedmolecular weight: 46.3 kDa). Specific band detection by Western blot is blocked bypeptide competition by pre-incubating the antibody with the immunizing peptide prior toreaction with the membrane. Reactivity in other immunoassays is unknown.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.

Détails sur RAD52

Antigène: RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

Autre désignation: RAD52 (RAD52 Produits)

Synonymes: anticorps Rad52yh, anticorps RAD52, anticorps zgc:112400, anticorps LOC100223085, anticorps MGC115453, anticorps RAD52 homolog, DNA repair protein, anticorps RAD52 homolog (S. cerevisiae), anticorps RAD52 homolog, DNA repair protein S homeolog, anticorps recombinase, anticorps DNA repair and recombination protein, putative, anticorps RAD52 protein, anticorps RAD52, anticorps Rad52, anticorps rad52, anticorps rad52.S

Sujet: The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. Four alternatively spliced transcript variants encoding different isoforms have been reported for this gene. The alpha variant encodes the longest isoform that contains an identical N-terminus, but a distinct C-terminus, as compared to other isoforms (beta, delta, and gamma).Synonyms: DNA repair protein RAD52 homolog

ID gène: 5893

NCBI Accession: NP_602296

UniProt: P43351

Pathways: Réparation de l'ADN