DCLRE1C anticorps (AA 482-495)

N° du produit ABIN117935

L’anticorps Chèvre Polyclonal anti-DCLRE1C a été validé pour WB, IHC (p) et EIA. Il convient pour détecter DCLRE1C dans des échantillons de Humain.

Aperçu rapide pour DCLRE1C anticorps (AA 482-495) (ABIN117935)

Antigène: DCLRE1C (DNA Cross-Link Repair 1C (DCLRE1C))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp DCLRE1C est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-DCLRE1C anticorps

Épitope: AA 482-495

Specificité: This antibody detects a band of the appropriate size in a number of different cell lines. However, it does not detect a band in cell lines that do not express ARTEMIS, such as the cell line CJ and in an hTERT line.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase.

Immunogène: This antibody was prepared from whole Goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 482-495 of Human ARTEMIS (DCLRE1C DNA cross- link repair 1C). Genename: DCLRE1C

Information d'application

Indications d'application: This antibody has been tested for use in ELISA (1: 10,000-1: 20,000), Immunohistochemistry(1: 1,000) and Western blotting (1: 500-1: 2,000). Reactivity in other immunoassays isunknown. This antibody detects a band of approximately 90 kDa (predicted molecular weight: 78 kDa).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.2 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, with 0.01 %(w/v) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.

Détails sur DCLRE1C

Antigène: DCLRE1C (DNA Cross-Link Repair 1C (DCLRE1C))

Autre désignation: Artemis

Sujet: Artemis has a role in T and B lymphocyte immunodeficiency and in predisposition to lymphoma through the NHEJ pathway of DNA repair. DNAPKcs regulates Artemis by both phosphorylation and complex formation to permit enzymatic activities that are critical for the hairpin-opening step of V(D)J recombination and for the 5' and 3' overhang processing in non-homologous DNA end joining.Synonyms: A-SCID protein, ASCID, DCLRE1C, DNA cross-link repair 1C protein, SCIDA, SNM1-like protein, SNM1C

ID gène: 64421

NCBI Accession: NP_001029027

UniProt: Q96SD1

Pathways: Réparation de l'ADN