PLK1 anticorps (pThr210)

N° du produit ABIN117982

Détail du produit anti-PLK1 anticorps

Antigène: PLK1 (Polo-Like Kinase 1 (PLK1))

Épitope: AA 205-214, pThr210

Reactivité: Humain, Souris, Rat, Chien, Poisson zèbre (Danio rerio), Porc, Xenopus laevis, Poulet, Chimpanzé

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PLK1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Séquence: 1 msaavtagkl arapadpgka gvpgvaapga paaappakei pevlvdprsr rryvrgrflg 61 kggfakcfei sdadtkevfa gkivpkslll kphqrekmsm eisihrslah qhvvgfhgff 121 edndfvfvvl elcrrrslle lhkrrkalte pearyylrqi vlgcqylhrn rvihrdlklg 181 nlflnedlev kigdfglatk veydgerkkt lcgtpnyiap evlskkghsf evdvwsigci 241 mytllvgkpp fetsclkety lrikkneysi pkhinpvaas liqkmlqtdp tarptinell 301 ndefftsgyi parlpitclt ipprfsiaps sldpsnrkpl tvlnkglenp lperprekee 361 pvvretgevv dchlsdmlqq lhsvnaskps erglvrqeea edpacipifw vskwvdysdk 421 yglgyqlcdn svgvlfndst rlilyndgds lqyierdgte syltvsshpn slmkkitllk 481 yfrnymsehl lkaganitpr egdelarlpy lrtwfrtrsa iilhlsngsv qinffqdhtk 541 lilcplmaav tyidekrdfr tyrlslleey gcckelasrl ryartmvdkl lssrsasnrl 601 kas

Specificité: This antibody is specific for phosphorylated Human Plk-1 protein at the pThr210 residue. Cross reactivity with Plk-1 protein homologues from C.elgans and Honeybee may also occur as sequence homology varies by one amino acid residue in this sequence. Reactivity with Plk-1 protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.

Réactivité croisée (Details): Species reactivity (expected):Chimpanzee, Pig, Chicken, Mouse, Rat, Xenopus, Dog, Mosquito, Zebrafish, Starfish, Sea urchin and Fruit Fly.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities

Immunogène: Synthetic phospho peptide corresponding aa 205-214 of Human Polo-like kinase 1 (Plk1) protein

Information d'application

Indications d'application: ELISA (1/3,000-1/12,000). Immunohistochemistry on Paraffin Sections (1/200-1/1,000). This antibody is positive byIHC on kidney, liver, cancer, thyroid and lymphocyte tissue. Western blot (1/200-1/2,000). Expect a band approximately 68 kDa in size correspondingto Plk-1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur PLK1

Antigène: PLK1 (Polo-Like Kinase 1 (PLK1))

Autre désignation: PLK1 (PLK1 Produits)

Synonymes: anticorps PLK, anticorps STPK13, anticorps plk, anticorps PLK-1, anticorps Plx1, anticorps stpk13, anticorps Plk, anticorps cb525, anticorps cb636, anticorps ik:tdsubc_2d1, anticorps wu:fb37g11, anticorps wu:fb76g03, anticorps xx:tdsubc_2d1, anticorps polo like kinase 1, anticorps polo like kinase 1 S homeolog, anticorps polo-like kinase 1, anticorps polo-like kinase 1 (Drosophila), anticorps Serine/threonine-protein kinase plk-1, anticorps PLK1, anticorps plk1.S, anticorps plk1, anticorps Plk1, anticorps plk-1

Sujet: Polo-like kinase 1, also known as Plk-1, serine-threonine protein kinase 13 and STPK13, may be required for cell division and have a role during G1 or S phase and is associated with the phosphorylation of cyclin B1. Phosphorylation of Plk-1 on T210 contributes to proper mitotic progression. Plk-1 has a nuclear localization and accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase. Plk-1 is a member of the Ser/Thr protein kinase family and the CDC5/Polo subfamily and contains 2 POLO box domains.Synonyms: PLK-1, Polo-like kinase 1, STPK13, Serine/threonine-protein kinase PLK1

ID gène: 5347

NCBI Accession: NP_005021

UniProt: P53350

Pathways: Cycle Cellulaire, M Phase