Thyroglobulin anticorps

N° du produit ABIN118049

L’anticorps anti-Thyroglobulin Monoclonal Souris est utilisé pour la détection de Thyroglobulin dans des échantillons de Humain, Souris, Rat et Chien. Il a été validé pour WB, ELISA, IHC (p), IP et IHC (fro).

Aperçu rapide pour Thyroglobulin anticorps (ABIN118049)

Antigène: Thyroglobulin (TG)

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Thyroglobulin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 2H11

Détail du produit anti-Thyroglobulin anticorps

Specificité: This protein A purified mouse monoclonal antibody reacts specifically with thyroglobulin in human tissues. The antibody recognizes a 330-kDa band corresponding to thyroglobulin. Cross reactivity with thyroglobulin from mouse, rat and dog will occur. Cross reactivity with thyroglobulin from other sources has not been determined.

Purification: Protein A chromatography.

Immunogène: This protein A purified monoclonal antibody was produced by repeated immunizations with human thyroglobulin protein. Produced by the fusion between BALB/c mouse splenocytes and mouse myeloma SP2/0 cells after immunization with thyroglobulin from human thyroid gland using conventional hybridoma technology.

Isotype: IgG1

Information d'application

Indications d'application: Suitable for the detection of thyroglobulin in ELISA, Immunohistochemistry,Immunoblotting and Immunoprecipitation. For Immunohistochemistry, both frozen sections and formalin fixed, paraffin-embeddedtissue sections can be used without epitope retrieval or enzyme digestion. This antibody isspecific for the 330 kDa thyroglobulin protein. Thyroglobulin shows a cytoplasmiclocalization. Thyroid tissue can be used as a positive control. Generally a 1: 50 to 1: 200dilution is suitable for most applications. A 30 to 60 minute incubation time is usuallysufficient. However, depending upon the fixation conditions and the staining systememployed, optimal incubation should be determined by the user. Both frozen sections andformalin fixed, paraffin embedded tissues are recommended for preparations to use forImmunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.6 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 2-8°C.

Date de péremption: 12 months

Détails sur Thyroglobulin

Antigène: Thyroglobulin (TG)

Sujet: Thyroglobulin (Tg) is synthesized by the follicular epithelial cells of the thyroid and secreted from the thyroid gland with the stimulation of TSH and/or thyroid stimulating immunoglobulins. Thyroglobulin is a prognostic marker for Graves` disease. Thyroglobulin antibody has been useful in the positive identification of thyroid carcinomas of the papillary and follicular types.
Alternate names: TGN, Thyreoglobulin

ID gène: 7038

NCBI Accession: NP_003226

UniProt: P01266

Pathways: Thyroid Hormone Synthesis