ATG7 anticorps (AA 558-569)

N° du produit ABIN118061

L’anticorps Lapin Polyclonal anti-ATG7 a été validé pour WB et EIA. Il convient pour détecter ATG7 dans des échantillons de Humain. Il y a 2+ publications disponibles.

Aperçu rapide pour ATG7 anticorps (AA 558-569) (ABIN118061)

Antigène: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATG7 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-ATG7 anticorps

Épitope: AA 558-569

Specificité: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human Apg7 protein. However, BLAST analysis indicates 100 % homology (12/12) for this protein from human, chimpanzee, mouse, rat, dog, and chicken sources. The core amino acids of the immunogen, G-D-S-T-R-D-R-T are 100 % identical (8/8) in D. melanogaster. Reactivity with Apg7 proteins from other sources is not known.

Purification: Immunoaffinity chromatography

Immunogène: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 558-569 of Human Apg7 protein.

Information d'application

Indications d'application: This affinity purified antibody has been tested for use in ELISA (1: 15,000-1: 75,000) and byWestern blot (1: 500- 1: 2,000). Specific conditions for reactivity should be optimized by theend user. Expect a band approximately 78 kDa in size corresponding to human Apg7 bywestern blotting in the appropriate cell lysate or extract. Human Apg7 has been reported toform a homodimer at approximately 160 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.46 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Dilute only prior to immediate use

Stock: -20 °C

Stockage commentaire: Store vial at -20° C prior to opening. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.

Références pour anticorps anti-ATG7 (ABIN118061)

Aoki, Kobayashi, Tanida, Hatano, Komori, Matsumoto, Nishioka, Uemura: "[A difficult case of esophageal and gastric double cancer with pleural and pericardial effusion following chemo-radiotherapy (CRT)]" dans: Gan to kagaku ryoho. Cancer & chemotherapy, Vol. 34, Issue 12, pp. 1979-81, (2008) (PubMed).

Fu, Yuan, Sun, Cui: "[Relation between acute respiratory infection and enterovirus in children in Beijing area.]" dans: Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology, Vol. 21, Issue 4, pp. 316-8, (2008) (PubMed).

Détails sur ATG7

Antigène: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Autre désignation: APG7L / ATG7

Sujet: Apg7 is the human gene product similar in homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae Apg7. In the yeast, the protein appears to be required for fusion of peroxisomal and vaculuolar membranes. The protein shows homlogy to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.Synonyms: APG7-like, Autophagy-related protein 7, Ubiquitin-activating enzyme E1-like protein

ID gène: 10533

NCBI Accession: NP_001129503

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagy