This antibody recognises the Mouse CD71 marker, the transferrin receptor. Stains dividing cells, and can be used to distinguish resting from activated T cells.
Purification
Ion Exchange Chromatography.
Immunogène
Concanavilin A activated mouse spleen cells. Spleen cells from immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.1 mg/mL
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
CD71 antigen expression is low on normal resting lymphocytes and is expressed on all cells upon activation. The transferrin receptor is essential for iron transport into proliferating cells, such as mitogen-activated and alloantigen-activated lymphoblasts. The transferrin receptor is also present on early erythroid cells but is lost as reticulocytes differentiate into mature erythrocytes.Synonyms: TfR1, Transferrin receptor protein 1, p90