CD16 anticorps

N° du produit ABIN118541

L’anticorps Souris Monoclonal anti-CD16 a été validé pour FACS et IP. Il convient pour détecter CD16 dans des échantillons de Humain.

Aperçu rapide pour CD16 anticorps (ABIN118541)

Antigène: CD16

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD16 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: LNK16

Détail du produit anti-CD16 anticorps

Specificité: This antibody is a 50-65 kDa cell surface molecule which exists in two forms - a transmembranous form expressed by NK cells and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. This antibody has been reported to block the binding of IgG to the CD16 Molecule (Ref.4)

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Ion Exchange Chromatography

niveau d'endotoxine: Low Endotoxin

Immunogène: Normal Human peripheral blood granulocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: 1/100 - 1/200, use 10 μL of suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01Eu/ug

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, None, None

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur CD16

Antigène: CD16

Autre désignation: CD16

Sujet: CD16 (FcgammaRIII) serves as a low affinity IgG receptor. Human CD16 is expressed in two forms CD16a and CD16b. CD16a is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. CD16b is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.Synonyms: CD16A, FCG3, FCGR3, FCGR3A, Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FcR-10, FcRIII, FcRIIIa, IGFR3, IgG Fc receptor III-2, Low affinity immunoglobulin gamma Fc region receptor III-A

ID gène: 2214

NCBI Accession: NP_000560

UniProt: P08637

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process