CD55 anticorps

N° du produit ABIN118737

Cet anticorps anti-CD55 est un anticorps Souris Monoclonal détectant CD55 dans FACS et IHC (fro). Adapté pour Humain. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour CD55 anticorps (ABIN118737)

Antigène: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD55 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 67

Détail du produit anti-CD55 anticorps

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G.

Immunogène: K562 cells

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: Use 10 μL of 1/10-1/25 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections: 1/100-1/1000. Recommended Positive Control Tissue: Human Tonsil. (Successful use in immunohistochemistry on paraffin embedded sections (1/50-1/100using heat treatment for antigen retrieval) was reported by some researchers. As thisdetection method has not been verified by antibodies-online, the application was deletedfrom the database. This does not necessarily exclude the use in such procedure).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, with 0.09 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-CD55 (ABIN118737)

Wenzel, Zabojszcza, Carl, Taubert, Lass, Harris, Ho, Schulz, Hummel, Hubner, Osterziel, Spuler: "Increased susceptibility to complement attack due to down-regulation of decay-accelerating factor/CD55 in dysferlin-deficient muscular dystrophy." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 9, pp. 6219-25, (2005) (PubMed).

Détails sur CD55

Antigène: CD55 (Complement Decay-Accelerating Factor (CD55))

Autre désignation: CD55 / DAF

Sujet: CD55 is the complement regulatory protein, decay accelerating factor (DAF). It is a 70kD glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells the apparent molecular weight is somewhat larger. It has a substantial content of O-glycans, and also on N-glycan. DAF binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. DAF carries the Cromer related blood group antigens. DAF has a wide distribution on cells in non-haemopoietic tissues, particularly epithelium and is specifically found at the foetal-maternal interface in placenta. Soluble forms of DAF are found, for example, in plasma, saliva and urine. The antigen on erythrocytes is pronase and chymotrypsin sensitive, but resistant to trypsin. CD55 is expressed by a wide range of cell types.Synonyms: Complement decay-accelerating factor

ID gène: 9606

UniProt: P08174

Pathways: Système du Complément, Regulation of Leukocyte Mediated Immunity