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TPSAB1 anticorps

L’anticorps Souris Monoclonal anti-TPSAB1 a été validé pour WB, IHC (p), IF, IHC (fro) et EIA. Il convient pour détecter TPSAB1 dans des échantillons de Humain, Rat, Chien, Cat et Singe.
N° du produit ABIN118895

Aperçu rapide pour TPSAB1 anticorps (ABIN118895)

Antigène

Voir toutes TPSAB1 Anticorps
TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))

Reactivité

  • 63
  • 31
  • 14
  • 8
  • 5
  • 2
  • 1
  • 1
  • 1
Humain, Rat, Chien, Cat, Singe

Hôte

  • 69
  • 35
  • 2
  • 1
  • 1
Souris

Clonalité

  • 72
  • 36
Monoclonal

Conjugué

  • 58
  • 19
  • 7
  • 4
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp TPSAB1 est non-conjugé

Application

  • 79
  • 50
  • 35
  • 19
  • 17
  • 14
  • 10
  • 8
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone

AA1
  • Specificité

    This antiobdy recognises Mast Cell Tryptase, both alpha and beta isoforms. This antibody is an excellent marker for mast cells, and does not bind to any other cell type in immunohistology.

    Purification

    Affinity Chromatography on Protein G

    Immunogène

    Human mast cell tryptase purified from Human lung tissue. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

    Isotype

    IgG1
  • Indications d'application

    ELISA. Western Blot. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (1/10,000): This product requires either protein digestion pre-treatment of paraffin sections e.g. pronase, or HIER, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Tonsil.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS, pH 7.4, 0.09 % Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Antigène

    TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))

    Autre désignation

    Tryptase beta-1 (TPSAB1)

    Sujet

    Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Tryptase is considered to be an important marker of mast cell activation as well as an important mediator of inflammation.Synonyms: Mast Cell Tryptase, TPSB1, Tryptase I

    ID gène

    100507804

    NCBI Accession

    NP_003285

    UniProt

    Q15661
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