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Ubiquitin anticorps

Cet anticorps Souris Monoclonal détecte spécifiquement Ubiquitin dans WB, IHC (p), IHC (fro) et EIA. Il présente une réactivité envers Humain et Boeuf (Vache).
N° du produit ABIN118999

Aperçu rapide pour Ubiquitin anticorps (ABIN118999)

Antigène

Voir toutes Ubiquitin (Ub) Anticorps
Ubiquitin (Ub)

Reactivité

  • 171
  • 72
  • 72
  • 65
  • 20
  • 17
  • 17
  • 16
  • 14
  • 14
  • 13
  • 12
  • 11
  • 10
  • 9
  • 9
  • 9
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Humain, Boeuf (Vache)

Hôte

  • 108
  • 89
  • 3
  • 1
  • 1
Souris

Clonalité

  • 109
  • 93
Monoclonal

Conjugué

  • 81
  • 15
  • 11
  • 10
  • 10
  • 9
  • 7
  • 7
  • 7
  • 7
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Ubiquitin est non-conjugé

Application

  • 174
  • 111
  • 84
  • 59
  • 54
  • 41
  • 30
  • 30
  • 25
  • 21
  • 13
  • 13
  • 8
  • 3
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone

1B4-UB
  • Specificité

    This antibody recognises ubiquitin.

    Purification

    Affinity chromatography on Protein G

    Immunogène

    Ubiquitin from bovine erythrocytes conjugated to bovine gamma Globulin. Spleen cells from immunised BALB/c mouse were fused with cells of the mouse PAI myeloma cell line.

    Isotype

    IgG1
  • Indications d'application

    ELISA. Western Blot. Flow Cytometry: 1/10 - 1/50, Membrane permeabilisation is required for this application. Immunohistochemistry on frozen sections: 1/500 - 1/1000. Immunohistochemistry on paraffin sections: 1/500 - 1/1000, This product does not require protein digestion pre-treatment of paraffin sections, This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Positive Control: Human hypophysis.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS, pH 7.4 containing 0.09 % Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Antigène

    Ubiquitin (Ub)

    Autre désignation

    Ubiquitin

    Sujet

    Synonyms: Polyubiquitin-B, UBB

    ID gène

    7314

    UniProt

    P0CG47

    Pathways

    Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
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