HLAG anticorps

N° du produit ABIN119228

L’anticorps anti-HLAG Monoclonal Souris est utilisé pour la détection de HLAG dans des échantillons de Humain. Il a été validé pour WB et IHC (p).

Aperçu rapide pour HLAG anticorps (ABIN119228)

Antigène: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HLAG est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MEM-G-1

Détail du produit anti-HLAG anticorps

Specificité: The antibody reacts with denaturated HLA-G heavy chain. HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of trophoblast cells.

Purification: Affinity Chromatography on Protein-A

Immunogène: Recombinant Human HLA-G denaturated heavy chain

Isotype: IgG1

Information d'application

Indications d'application: Western Blotting. Immunohistochemistry (paraffin sections): 1:60 to 1:100, 1 h at RT. Positive tissue: Human placenta - extravillous cytotrophoblast. Staining technique: Standard ABC technique. Pretreatment: Heat retrieval in 0.01 M citrate buffer (4x2 min. in microwave oven).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4 with 15 mM Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to - 70 °C for longer.

Détails sur HLAG

Antigène: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Autre désignation: HLA Class I alpha G / HLA-G

Sujet: HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells.Synonyms: HLA class I histocompatibility antigen, HLA-6.0, HLAG, MHC class I antigen G

ID gène: 3135

NCBI Accession: NP_002118

UniProt: P17693

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response