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IL-4 anticorps

Cet anticorps anti-IL-4 est un anticorps Rat Monoclonal détectant IL-4 dans WB, FACS, EIA et IHC (fro). Adapté pour Humain.
N° du produit ABIN119373

Aperçu rapide pour IL-4 anticorps (ABIN119373)

Antigène

Voir toutes IL-4 (IL4) Anticorps
IL-4 (IL4) (Interleukin 4 (IL4))

Reactivité

  • 163
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Humain

Hôte

  • 123
  • 94
  • 89
  • 9
  • 3
  • 2
Rat

Clonalité

  • 195
  • 122
  • 2
Monoclonal

Conjugué

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  • 6
  • 4
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  • 1
Cet anticorp IL-4 est non-conjugé

Application

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Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone

MP4-25D2
  • Specificité

    This antibody is specific for Interleukin - 4 (IL-4). Clone MP4-25D2 is reported to neutralise IL-4 activity. We recommend the use of SM1702A for this purpose.

    Purification

    Affinity chromatography on Protein G

    Immunogène

    Purified CHO cell expressed recombinant human IL-4.

    Isotype

    IgG1
  • Indications d'application

    Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required for this application. ELISA. Western Blot: 1/500 - 1/1000. Immunohistochemistry on frozen sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS, pH 7.4 containing 0.09 % Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Antigène

    IL-4 (IL4) (Interleukin 4 (IL4))

    Autre désignation

    Interleukin-4 / IL4

    Sujet

    IL-4 is a 20kD cytokine which is expressed by a subpopulation of activated T cells and mast cells.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra

    ID gène

    3565

    NCBI Accession

    NP_000580

    UniProt

    P05112

    Pathways

    Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
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