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Aquaporin 1 anticorps (AA 249-269)

Cet anticorps anti-Aquaporin 1 est un anticorps Souris Monoclonal détectant Aquaporin 1 dans WB, IP, IHC (p), IHC (fro) et EIA. Adapté pour Humain, Rat et Souris.
N° du produit ABIN119568

Aperçu rapide pour Aquaporin 1 anticorps (AA 249-269) (ABIN119568)

Antigène

Voir toutes Aquaporin 1 (AQP1) Anticorps
Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Reactivité

  • 86
  • 66
  • 57
  • 5
  • 5
  • 4
  • 2
Humain, Rat, Souris

Hôte

  • 94
  • 11
  • 3
Souris

Clonalité

  • 87
  • 21
Monoclonal

Conjugué

  • 50
  • 10
  • 9
  • 6
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Aquaporin 1 est non-conjugé

Application

  • 83
  • 49
  • 39
  • 28
  • 24
  • 20
  • 17
  • 14
  • 14
  • 13
  • 7
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone

1-A5F6
  • Épitope

    • 16
    • 12
    • 11
    • 8
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 249-269

    Specificité

    This antibody recognises an epitope within the cytoplasmic domain of the water-specific channel Aquaporin 1.

    Purification

    Affinity Chromatography on Protein G.

    Immunogène

    Synthetic peptide corresponding to amino acids 249-269 of Rat Aquaporin 1. Spleen cells from immunised Balb/c mice were fused with the cells of the Sp2/0 Ag14 mouse myeloma cell line.

    Isotype

    IgG1
  • Indications d'application

    Immunoprecipitation: 1/1000 - 1/10000. Western Blot: 1/1000 - 1/5000. ELISA: 1/1000 - 1/20000. Immunohistochemistry on frozen and paraffin embedded sections: 1/500 - 1/1000. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended positive control tissue: Kidney.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Antigène

    Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

    Autre désignation

    Aquaporin-1

    Sujet

    Aquaporin 1 is a 28kD integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. The glycosylated forms of AQP1 range between 40-60kD in mass. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.Synonyms: AQP-1, AQP1, Aquaporin-CHIP, CHIP28, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule

    ID gène

    358

    NCBI Accession

    NP_932766

    UniProt

    P29972

    Pathways

    Hormone Transport
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