MCAM anticorps (N-Term) (FITC)

N° du produit ABIN119672

Il existe 1 publication pour ce produit. L’anticorps anti-MCAM Monoclonal Souris est utilisé pour la détection de MCAM dans des échantillons de Humain et Porc. Il a été validé pour FACS, IHC (fro) et EIA.

Aperçu rapide pour MCAM anticorps (N-Term) (FITC) (ABIN119672)

Antigène: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivité: Humain, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MCAM est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: OJ79c

Détail du produit anti-MCAM anticorps (FITC)

Épitope: AA 1-5, N-Term

Specificité: This antibody recognises the 118 kDa cell surface glycoprotein CD146, also known as MUC18, Mel-CAM and S-endo. CD146 is a member of the immunoglobulin superfamily, expressed by all endothelial cells and by melanoma cells.

Purification: Affinity Chromatography on Protein G

Immunogène: Recombinant Human MUC18 (D1-D5) Fc protein. Spleen cells from immunised mice were fused with cells of the mouse Sp2/0 Ag.14 myeloma cell line.

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: neat - 1/10, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Clone OJ79c (purified antibody) has been successfully used in ELISA and immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, 0.09 % Sodium Azide, 1 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-MCAM (FITC) (ABIN119672)

Möbius-Winkler, Hilberg, Menzel, Golla, Burman, Schuler, Adams: "Time-dependent mobilization of circulating progenitor cells during strenuous exercise in healthy individuals." dans: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 107, Issue 6, pp. 1943-50, (2010) (PubMed).

Détails sur MCAM

Antigène: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Autre désignation: CD146

Sujet: CD146 is a member of the immunoglobulin superfamily, expressed by all endothelial cells and by melanoma cells. CD146 appears to act as an adhesion molecule. Expression in melanoma may be linked to disease progression.Synonyms: A32, CD146, Cell surface glycoprotein P1H12, MCAM, MUC18, Melanoma cell adhesion molecule, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen

ID gène: 4162

NCBI Accession: NP_006491

UniProt: P43121