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CD31 anticorps

L’anticorps Souris Monoclonal anti-CD31 a été validé pour FACS, WB, IHC (fro) et EIA. Il convient pour détecter CD31 dans des échantillons de Rat.
N° du produit ABIN120452

Aperçu rapide pour CD31 anticorps (ABIN120452)

Antigène

Voir toutes CD31 (PECAM1) Anticorps
CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivité

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Rat

Hôte

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Souris

Clonalité

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Monoclonal

Conjugué

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Cet anticorp CD31 est non-conjugé

Application

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Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone

TLD-3A12
  • Specificité

    This antibody recognises PECAM-1 (CD31). Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen.

    Purification

    Affinity Chromatography on Protein G

    niveau d'endotoxine

    Low Endotoxin

    Immunogène

    Activated, Lewis Rat derived microglial cells. Remarks: Spleen cells from immunised BALB/c mouse were fused with cells of the mouse SP2 myeloma cell line.

    Isotype

    IgG1
  • Indications d'application

    ELISA. Western Blot. Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS without preservatives or stabilizers

    Agent conservateur

    Without preservative

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • Antigène

    CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

    Autre désignation

    CD31 / PECAM1

    Sujet

    CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets. CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas). CD31 is a type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.Synonyms: End °CAM, GPIIA', PECAM-1, Platelet endothelial cell adhesion molecule

    ID gène

    29583

    NCBI Accession

    NP_113779

    UniProt

    Q3SWT0

    Pathways

    Regulation of Actin Filament Polymerization
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