CD31 anticorps

N° du produit ABIN120453

Détail du produit anti-CD31 anticorps

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD31 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificité: This antibody recognizes PECAM-1 (CD31). Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen (3). SM272LE is recommended for use in Functional Studies.

Aucune reactivité croisée: Humain

Réactivité croisée (Details): Species reactivity (tested):Rat.

Purification: Affinity C Chromatography on Protein G

Immunogène: Activated, Lewis rat derived microglial cells. Spleen cells from immunised BALB/c mouse were fused with cells of the mouse SP2 myeloma cell line.

Clone: TLD-3A12

Isotype: IgG1

Information d'application

Indications d'application: ELISA. Western Blot. Flow Cytometry: 1/10 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections: 1/10 - 1/100. Does not work on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CD31

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Autre désignation: CD31 / PECAM1 (PECAM1 Produits)

Synonymes: anticorps CD31, anticorps CD31/EndoCAM, anticorps GPIIA', anticorps PECA1, anticorps PECAM-1, anticorps endoCAM, anticorps C85791, anticorps Cd31, anticorps Pecam, anticorps platelet/endothelial cell adhesion molecule 1, anticorps platelet and endothelial cell adhesion molecule 1, anticorps pecam1, anticorps PECAM1, anticorps Pecam1

Sujet: CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets. CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas). CD31 is a type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.Synonyms: End °CAM, GPIIA', PECAM-1, Platelet endothelial cell adhesion molecule

ID gène: 29583

NCBI Accession: NP_113779

UniProt: Q3SWT0

Pathways: Regulation of Actin Filament Polymerization