CD34 anticorps

N° du produit ABIN120583

Cet anticorps Souris Monoclonal détecte spécifiquement CD34 dans FACS, WB et IHC (p). Il présente une réactivité envers Humain.

Aperçu rapide pour CD34 anticorps (ABIN120583)

Antigène: CD34

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD34 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 4H11

Détail du produit anti-CD34 anticorps

Specificité: The antibody reacts with Class III epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells, it is gradually lost on progenitor cells. The antibody completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line.

Réactivité croisée (Details): Species reactivity (tested):Human

Purification: Precipitation methods

Immunogène: Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry. Western Blotting (non-reducing conditions): 2 μg/mL. Positive control: Kg-1a human leukemia cell lysate. Negative control: JURKAT human leukemia T-cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. Immunohistochemistry-Paraafin: Recommended dilution: 10 m g/mL. Positive tissue: placenta endothelium.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.4 with 15 mM Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Date de péremption: 12 months

Détails sur CD34

Antigène: CD34

Autre désignation: CD34

Sujet: CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker

ID gène: 947

UniProt: P28906