CD22 anticorps
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- Antigène Voir toutes CD22 Anticorps
- CD22 (CD22 Molecule (CD22))
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Reactivité
- Humain, Primate
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD22 est non-conjugé
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Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)
- Réactivité croisée (Details)
- Species reactivity (tested):Human, non-human Primates
- Purification
- Protein-A affinity chromatography
- Pureté
- > 95 % pure (by SDS-PAGE)
- Immunogène
- Raji Burkitt's lymphoma cell line
- Clone
- MEM-01
- Isotype
- IgG1
- Top Product
- Discover our top product CD22 Anticorps primaire
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- Indications d'application
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Flow cytometry. Immunoprecipitation. Western blotting: Recommended dilution: 2-4 μg/mL, 120 min on vertical incubator Positive control: RAJI human lymphoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
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Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. - Date de péremption
- 12 months
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- Antigène
- CD22 (CD22 Molecule (CD22))
- Autre désignation
- CD22 (CD22 Produits)
- Synonymes
- anticorps A530093D23, anticorps Lyb-8, anticorps Lyb8, anticorps SIGLEC-2, anticorps SIGLEC2, anticorps siglec-2, anticorps CD22 antigen, anticorps CD22 molecule, anticorps Cd22, anticorps CD22
- Sujet
- CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.Synonyms: B-cell receptor CD22, B-lymphocyte cell adhesion molecule, BL-CAM, Leu-14, SIGLEC2, Sialic acid-binding Ig-like lectin 2, Siglec-2
- UniProt
- P20273
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