MHC Class I RT1.Aa alpha-Chain (RT1.AA) anticorps

N° du produit ABIN120721

Cet anticorps Souris Monoclonal détecte spécifiquement MHC Class I RT1.Aa alpha-Chain dans FACS, IHC (fro) et IP. Il présente une réactivité avec des échantillons de Rat.

Aperçu rapide pour MHC Class I RT1.Aa alpha-Chain (RT1.AA) anticorps (ABIN120721)

Antigène: MHC Class I RT1.Aa alpha-Chain (RT1.AA)

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Clone: OX-18

Détail du produit

Purification: Protein G affinity chromatography

Immunogène: Rat spleen cell glycoprotein

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: 1:50-1:100, use 10 μL of the suggested working dilution to label 10^6 cells. Immunohistochemistry on frozen sections: Acetone fixation recommended - the antigen is sensitive to fixation with paraformaldehyde. Not suitable on paraffin embedded sections. ELISA. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % sodium azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2-8 °C for up to one month or at -20 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: one year from despatch.

Date de péremption: 12 months

Détail du antigène

Antigène: MHC Class I RT1.Aa alpha-Chain (RT1.AA)

Autre désignation: MHC Class I (RT1Aa)

Sujet: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: RT1 class I histocompatibility antigen AA alpha chain