LYN anticorps (AA 8-238)

N° du produit ABIN120725

Il existe 3+ publications pour ce produit. L’anticorps anti-LYN Monoclonal Souris est utilisé pour la détection de LYN dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IP et IF.

Aperçu rapide pour LYN anticorps (AA 8-238) (ABIN120725)

Antigène: LYN (V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LYN est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: LYN-01

Détail du produit anti-LYN anticorps

Épitope: AA 8-238

Specificité: The antibody reacts with Lyn (p56/p53), a non-receptor Src-family tyrosine kinase expressed in hematopoietic tissues.

Purification: Protein A affinity chromatography

Immunogène: Bacterially expressed recombinant fragment of human Lyn (aa 8 - 238)

Isotype: IgG1

Information d'application

Indications d'application: Immunoprecipitation. Immunocytochemistry. Western Blotting: Recommended dilution: 1-2 μg/mL, 60 min. Positive control: RBL rat basophilic leukemia cell line JURKAT human peripheral blood leukemia T-cell line A-431 human epidermoid carcinoma cell line U-937 human histiocytic lymphoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate (1:1) with non- reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (12 % separating gel).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Date de péremption: 12 months

Références pour anticorps anti-LYN (ABIN120725)

Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." dans: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, (2002) (PubMed).

Kovárová, Tolar, Arudchandran, Dráberová, Rivera, Dráber: "Structure-function analysis of Lyn kinase association with lipid rafts and initiation of early signaling events after Fcepsilon receptor I aggregation." dans: Molecular and cellular biology, Vol. 21, Issue 24, pp. 8318-28, (2001) (PubMed).

Amoui, Dráberová, Tolar, Dráber: "Direct interaction of Syk and Lyn protein tyrosine kinases in rat basophilic leukemia cells activated via type I Fc epsilon receptors." dans: European journal of immunology, Vol. 27, Issue 1, pp. 321-8, (1997) (PubMed).

Détails sur LYN

Antigène: LYN (V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN))

Autre désignation: LYN

Sujet: Lyn (also known as p53/56 Lyn) is a membrane associated protein tyrosine kinase (PTK) mostly expressed in hemopoietic cells which is important in cellular signaling. It contains an SH2 and SH3 domain and has been found to be cleaved after activation of caspases in apoptosis. A member of the Src family of PTKs, there are two known isoforms for Lyn which plays an indispensable role in the Fc epsilon RI (Fcer1) and the B cell IgM receptor signaling pathway and is essential for Syk activation and Lat phosphorylation after Fcer1 aggregation and can also phosphorylate Tec on multiple residues. Lyn can also be regulated by IL2 and IL3. Lyn is a member of the src family of non-receptor protein tyrosine kinases that is predominantly expressed in haematopoietic tissues. Like all members of the src family, lyn is thought to participate in signal transduction from cell surface receptors that lack intrinsic tyrosine kinase activity. It is associated with a number of cell surface receptors including the B cell antigen receptor and immunoglobulin E receptor (FceRI).Synonyms: Tyrosine-protein kinase Lyn

ID gène: 4067

UniProt: P07948

Pathways: Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling, Integrin Complex, BCR Signaling