beta Amyloid anticorps (C-Term, N-Term)

N° du produit ABIN126725

L’anticorps anti-beta Amyloid Monoclonal Souris est utilisé pour la détection de beta Amyloid dans des échantillons de Humain. Il a été validé pour WB, IF et EIA.

Aperçu rapide pour beta Amyloid anticorps (C-Term, N-Term) (ABIN126725)

Antigène: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp beta Amyloid est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Clone: 8G7

Détail du produit anti-beta Amyloid anticorps

Épitope: AA 1-42, C-Term, N-Term

Specificité: This antibody specifically interacts with the C-Terminus of beta-Amyloid (1-42) and does not crossreact with beta-Amyloid (1-40).

Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography

Immunogène: C-terminal peptide (aa 1-42) of Amyloid beta A4 - conjugated to KLH

Isotype: IgG1

Information d'application

Indications d'application: ELISA: 0.05 μg/mL. Immunoblotting: 1.0 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween20 based blocking and blot incubation buffer. Immunocytochemistry: 0.1-1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1.0 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS/0.09 % Sodium Azide/PEG and Sucrose

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -20 °C to -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may bestored at 2-8 °C up to 3 months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur beta Amyloid

Antigène: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Autre désignation: Amyloid beta

Sujet: The beta-amyloid peptide (beta A4), proteolytically released from the amyloid precursor protein (APP), is the principal component of senile plaques in Alzheimer's disease. Cleavage of APP by alpha-secretase or alternatively by beta-secretase leads to generation and extracellular release of soluble APP peptides, S-APP-alpha and S-APP-beta, respectively, and the retention of corresponding membrane-anchored C-terminal fragments, C83 and C99. Subsequent processing of C83 by gamma-secretase yields P3 peptides. This is the major secretory pathway and is nonamyloidogenic. Alternatively, presenilin/nicastrin-mediated gamma-secretase processing of C99 releases the amyloid beta proteins, amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42), major components of amyloid plaques, and the cytotoxic C-terminal fragments, gamma-CTF(50), gamma-CTF(57) and gamma-CTF(59).Synonyms: Amyloid beta peptide

Pathways: Inflammasome