CK1 epsilon anticorps (AA 355-380)

N° du produit ABIN126752

L’anticorps anti-CK1 epsilon Monoclonal Souris est utilisé pour la détection de CK1 epsilon dans des échantillons de Humain, Souris, Rat et Chien. Il a été validé pour WB et EIA.

Aperçu rapide pour CK1 epsilon anticorps (AA 355-380) (ABIN126752)

Antigène: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CK1 epsilon est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 4D7

Détail du produit anti-CK1 epsilon anticorps

Épitope: AA 355-380

Specificité: Mab CK1e-4D7 specifically recognizes the epsilon isoform of CK1.

Attributs du produit: Synonyms: CKI-epsilon, CKIe, CSNK1E

Purification: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.

Ingrédients: incl. positive Control

Immunogène: Peptide (aa 355 - 380 of CK1e) conjugated to hemocyanin

Isotype: IgG1

Information d'application

Indications d'application: ELISA (0.1 μg/mL). Western blot (detects a band at 45 kD, Use at 0.5 μg/mL for HRPO/ECL detection,Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: For positive control cell lysate from serum starved A431 cells.

Restrictions: For Research Use only

Stockage

Reconstitution: Reconstitute with 1 mL H2O (15 min, RT)

Buffer: 2 x PBS / 0.09 % Na-azide / PEG and Sucrose

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: For long term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution aliquoteand freeze in liquid nitrogen, reconstituted antibody can be stored frozen at -80 °C up to 1year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months. Avoidrepeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur CK1 epsilon

Antigène: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Autre désignation: Casein Kinase I epsilon

Sujet: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as subtrates. It can also phosphorylate a large number of proteins and participates in Wnt signaling.Synonyms: CKI-epsilon, CKIe, CSNK1E

ID gène: 1454

UniProt: P49674

Pathways: Signalisation Hedgehog, M Phase