YES1 anticorps (N-Term)

N° du produit ABIN126915

Cet anticorps anti-YES1 est un anticorps Souris Monoclonal détectant YES1 dans WB et EIA. Adapté pour Humain.

Aperçu rapide pour YES1 anticorps (N-Term) (ABIN126915)

Antigène: YES1 (V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp YES1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 2B8

Détail du produit anti-YES1 anticorps

Épitope: N-Term

Specificité: AM00159PU-N specifically recognizes Yes at 59 kDa.

Attributs du produit: Synonyms: YES, p61-Yes, c-Yes, Proto-oncogene tyrosine-protein kinase Yes

Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

Ingrédients: incl. positive Control

Immunogène: Synthetic peptide conjugated to KLH

Isotype: IgG1

Information d'application

Indications d'application: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Use A431 untreated cell lysate as Positive Control. ELISA: 0.05 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Lyophilized Cell Lysate from serum starved A431 cellsReconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control is recommended for immunoblot applications. 20 µl of positive controlcell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation!

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS / 0.09 % Sodium Azide / PEG and Sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.

Date de péremption: 12 months

Détails sur YES1

Antigène: YES1 (V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1))

Autre désignation: YES1

Sujet: Yes is a member of a family of 9 tyrosine kinases regulating cellular responses to extracellular stimuli. Yes, src and fyn are ubiquitously expressed while the other members are primarily expressed in hematopoietic cells. Each protein tyrosine kinase (PTK) contains three domain (SH1, SH2, SH3). The SH1 domain has PTK activity, while the SH2 and SH3 domains are invloved in mediating protein-protein interactions by binding phosphotyrosine- containing and proline-riche motifs. By means of SH2-phosphotyrosine binding, PTKs are able to associate with activated receptor protein tyrosine kinases, e.g. platelet-derived growth factor (PDGFR)- and epidermal growth factor (EGF)- receptor.Synonyms: Proto-oncogene tyrosine-protein kinase Yes, YES, c-Yes, p61-Yes

ID gène: 7525

UniProt: P07947

Pathways: CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling