BMI1 anticorps (AA 252-264)

N° du produit ABIN129562

N° du produit (Fournisseur): 600-101-392

Cet anticorps Chèvre Polyclonal détecte spécifiquement BMI1 dans WB, ELISA, IF, FM et MA. Il présente une réactivité envers Humain.

Aperçu rapide pour BMI1 anticorps (AA 252-264) (ABIN129562)

Antigène: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp BMI1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Détail du produit anti-BMI1 anticorps

Épitope: AA 252-264

N° du produit (Fournisseur): 600-101-392

Fournisseur: Rockland

Fonction: BMI1 Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against human Bmi1 protein.

Attributs du produit: Synonyms: goat anti-Bmi-1 antibody, Bmi1, B lymphoma Mo MLV insertion region antibody, Bmi 1 antibody, MGC12685 antibody, Murine leukemia viral (bmi 1) oncogene homolog antibody, Oncogene BMI 1 antibody, PCGF 4 antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 252-264 of human Bmi1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified antibody has been tested for use in ELISA, immunofluorescence, and western blot.  A 1:200 dilution is reported to be effective for Immunofluorescence against Methanol fixed cells. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kDa in size corresponding to Bmi1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:30,000

IF Microscopy Dilution: 1:200

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.91 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur BMI1

Antigène: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Autre désignation: BMI1

Sujet: Background: The Bmi-1 oncogene (also known as polycomb group ring finger 4, MGC12685, murine leukemia viral (bmi 1) oncogene homolog, oncogene BMI 1, polycomb complex protein BMI 1 and RNF51) induces telomerase activity and immortalizes human mammary epithelial cells. Bmi-1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway.  The polycomb group (PcG) genes are involved in the maintenance of cellular memory through epigenetic chromatin modifications. Recent studies have implicated a role for PcG genes in the self-renewal of hematopoietic stem cells (HSCs), a process in which cellular memory is maintained through cell division. Among the PcG genes, Bmi-1 plays a central role in the inheritance of stemness, and its forced expression promotes HSC self-renewal. These findings highlight the importance of epigenetic regulation in HSC self-renewal and identify PcG genes as potential targets for therapeutic HSC manipulation.

ID gène: 648, 27883842

UniProt: P35226

Pathways: Cycle Cellulaire, Autophagy