Cyclin B1 anticorps (pSer126)

N° du produit ABIN129589

N° du produit (Fournisseur): 600-401-464

Cet anticorps Lapin Polyclonal détecte spécifiquement Cyclin B1 dans WB, ELISA et IP. Il présente une réactivité envers Humain et a été mentionné dans 1 publication.

Aperçu rapide pour Cyclin B1 anticorps (pSer126) (ABIN129589)

Antigène: Cyclin B1 (CCNB1)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cyclin B1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-Cyclin B1 anticorps

Épitope: pSer126

N° du produit (Fournisseur): 600-401-464

Fournisseur: Rockland

Fonction: Cyclin B1 phospho S126 Antibody

Réactivité croisée (Details): This antibody is specific for phosphorylated human Cyclin B1 protein at the pS126 residue.

Attributs du produit: Synonyms: rabbit anti-Cyclin B1 pS126 Antibody, Cyclin B1, CCNB1, CCNB, G2/mitotic-specific cyclin-B1

Purification: This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 115-140 of Human Cyclin B1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.   Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 48 kDa in size corresponding to Cyclin B1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:100 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 2.01 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-Cyclin B1 (ABIN129589)

Geng, Zhang, Zheng, Legerski: "Artemis links ATM to G2/M checkpoint recovery via regulation of Cdk1-cyclin B." dans: Molecular and cellular biology, Vol. 27, Issue 7, pp. 2625-35, (2007) (PubMed).

Détails sur Cyclin B1

Antigène: Cyclin B1 (CCNB1)

Autre désignation: CCNB1

Sujet: Background: Cyclin B1 (also called CCNB1 and G2/mitotic-specific cyclin B1) is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form a serine/threonine kinase holoenzyme complex called the maturation-promoting factor (MPF). Two alternative transcripts have been found a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.  S126 phosphorylation, the site of phosphorylation recognized by this antibody, is involved in an auto-phosphorylation of the cdk -cyclin complex.  Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.

ID gène: 891

UniProt: P14635

Pathways: Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase