SFRP1 anticorps

N° du produit ABIN129592

N° du produit (Fournisseur): 600-401-475

Cet anticorps Lapin Polyclonal détecte spécifiquement SFRP1 dans WB, IHC, ELISA et FM. Il présente une réactivité envers Humain et a été mentionné dans 3+ publications.

Aperçu rapide pour SFRP1 anticorps (ABIN129592)

Antigène: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SFRP1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Détail du produit anti-SFRP1 anticorps

N° du produit (Fournisseur): 600-401-475

Fournisseur: Rockland

Fonction: SFRP1 Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against human SFRP protein.

Attributs du produit: Synonyms: rabbit anti-SFRP1 antibody, SFRP-1, secreted frizzled related protein 1 antibody, FRP-1 antibody, FRP 1 antibody, FRP1 antibody, FrzA antibody, SARP 2 antibody, SARP2 antibody, Secreted Apoptosis-related Protein 2 antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène:

Immunogen: SFRP1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a 12 aa region of human Sfrp1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:800

Application Note: This affinity purified antibody has been tested for use in ELISA, western blot, and IHC.  This antibody is suitable for immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kDa in size corresponding to Sfrp1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:200-1:2,000

ELISA Dilution: 1:5,000 - 1:15,000

IF Microscopy Dilution: Use at a concentration of 5 μg/mL

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-SFRP1 (ABIN129592)

Huang, Zhang, Teng, Lin, Zheng, Yang, Han: "Down-regulation of SFRP1 as a putative tumor suppressor gene can contribute to human hepatocellular carcinoma." dans: BMC cancer, Vol. 7, pp. 126, (2007) (PubMed).

Garcia-Hoyos, Cantalapiedra, Arroyo, Esteve, Rodríguez, Riveiro, Trujillo, Ramos, Bovolenta, Ayuso: "Evaluation of SFRP1 as a candidate for human retinal dystrophies." dans: Molecular vision, Vol. 10, pp. 426-31, (2004) (PubMed).

Han, Amar: "Secreted frizzled-related protein 1 (SFRP1) protects fibroblasts from ceramide-induced apoptosis." dans: The Journal of biological chemistry, Vol. 279, Issue 4, pp. 2832-40, (2004) (PubMed).

Détails sur SFRP1

Antigène: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Autre désignation: SFRP1

Sujet: Background: Anti-SFRP1 is a Stem Cell Antibody. SFRP1 (also known as FRP, FRP1, SARP2, Secreted Apoptosis-related Protein 2, Secreted Frizzled-related Protein and Secreted frizzled-related protein 1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.

ID gène: 6422, 56117838

UniProt: Q8N474

Pathways: Signalisation WNT, Intracellular Steroid Hormone Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of fat Cell Differentiation