USP7 anticorps (N-Term)

N° du produit ABIN129596

N° du produit (Fournisseur): 600-401-482

L’anticorps Lapin Polyclonal anti-USP7 a été validé pour WB et ELISA. Il convient pour détecter USP7 dans des échantillons de Humain et Souris.

Aperçu rapide pour USP7 anticorps (N-Term) (ABIN129596)

Antigène: USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp USP7 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-USP7 anticorps

Épitope: N-Term

N° du produit (Fournisseur): 600-401-482

Fournisseur: Rockland

Fonction: HAUSP Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against human HAUSP protein.

Attributs du produit: Synonyms: rabbit anti-HAUSP antibody, USP7, Deubiquitinating enzyme 7 antibody, Herpes virus associated ubiquitin specific protease antibody, Ubiquitin carboxyl terminal hydrolase 7 antibody, Ubiquitin thioesterase 7, Ubiquitin-specific-processing protease 7

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of human HAUSP protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 130 kDa in size corresponding to HAUSP protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:15,000 - 1:60,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.98 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur USP7

Antigène: USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7))

Autre désignation: USP7

Sujet: Background: HAUSP (also known as deubiquitinating enzyme 7, herpes virus associated ubiquitin specific protease, TEF1, ubiquitin carboxyl terminal hydrolase 7, ubiquitin specific protease 7, ubiquitin thiolesterase 7, and USP7) is a novel p53 interacting protein. HAUSP was identified by mass spectrometry of affinity purified p53 associated factors by Li et al. HAUSP strongly stabilizes p53, even in the presence of excess MDM2, and also induces p53-dependent cell growth repression and apoptosis. HAUSP has an intrinsic enzymatic activity that specifically de-ubiquitinates p53 both in vivo and in vitro. Expression of a catalytically inactive point mutation of HAUSP in cells increased the levels of p53 ubiquitination and also destabilized p53. Li et al concluded that their findings revealed an important mechanism by which p53 can be stabilized by direct de-ubiquitination and also implied that HAUSP may function as a tumor suppressor in vivo through the stabilization of p53.

ID gène: 7874, 150378533

UniProt: Q93009