ATG7 anticorps (AA 550-575)

N° du produit ABIN129600

N° du produit (Fournisseur): 600-401-487

Cet anticorps anti-ATG7 est un anticorps Lapin Polyclonal détectant ATG7 dans WB, ELISA et FM. Adapté pour Humain. Ce Primary Antibody a été cité dans 7+ publications.

Aperçu rapide pour ATG7 anticorps (AA 550-575) (ABIN129600)

Antigène: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATG7 est non-conjugé

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Détail du produit anti-ATG7 anticorps

Épitope: AA 550-575

N° du produit (Fournisseur): 600-401-487

Fournisseur: Rockland

Fonction: APG7 Antibody

Réactivité croisée (Details): Reactivity occurs against human Apg7 protein.

Attributs du produit: Synonyms: rabbit anti-APG7 Antibody, APG-7, APG 7, APG7 autophagy 7-like antibody, APG7 like antibody, APG7L antibody, ATG 7 antibody, ATG-7 antibody, ATG7 autophagy related 7 homolog antibody

Purification: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 550-575 of Human Apg7 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 78 kDa in size corresponding to human Apg7 by western blotting in the appropriate cell lysate or extract.   Human Apg7 has been reported to form a homodimer at approximately 160 kDa.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:300,000 - 1:400,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.46 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-ATG7 (ABIN129600)

Bhattacharya, Parillon, Zeng, Han, Eissa: "Deficiency of autophagy in dendritic cells protects against experimental autoimmune encephalomyelitis." dans: The Journal of biological chemistry, Vol. 289, Issue 38, pp. 26525-32, (2015) (PubMed).

Abdel Fattah, Bhattacharya, Herron, Safdar, Eissa: "Critical role for IL-18 in spontaneous lung inflammation caused by autophagy deficiency." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 11, pp. 5407-16, (2015) (PubMed).

Fung, Chen, Grandis, Duvvuri: "EGFR tyrosine kinase inhibition induces autophagy in cancer cells." dans: Cancer biology & therapy, Vol. 13, Issue 14, pp. 1417-24, (2013) (PubMed).

Shin, Fattah, Bhattacharya, Ko, Eissa: "Inflammasome activation by altered proteostasis." dans: The Journal of biological chemistry, Vol. 288, Issue 50, pp. 35886-95, (2013) (PubMed).

Liu, Ko, Xu, Fattah, Xiang, Jagannath, Ishii, Komatsu, Eissa: "Transient aggregation of ubiquitinated proteins is a cytosolic unfolded protein response to inflammation and endoplasmic reticulum stress." dans: The Journal of biological chemistry, Vol. 287, Issue 23, pp. 19687-98, (2012) (PubMed).

Sarkar, Perlstein, Imarisio, Pineau, Cordenier, Maglathlin, Webster, Lewis, OKane, Schreiber, Rubinsztein: "Small molecules enhance autophagy and reduce toxicity in Huntington's disease models." dans: Nature chemical biology, Vol. 3, Issue 6, pp. 331-8, (2007) (PubMed).

Sarkar, Davies, Huang, Tunnacliffe, Rubinsztein: "Trehalose, a novel mTOR-independent autophagy enhancer, accelerates the clearance of mutant huntingtin and alpha-synuclein." dans: The Journal of biological chemistry, Vol. 282, Issue 8, pp. 5641-52, (2007) (PubMed).

Détails sur ATG7

Antigène: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Autre désignation: ATG7

Sujet: Background: Apg7 is the human gene product similar in homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae Apg7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.

ID gène: 10533

NCBI Accession: NP_001129503

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagy