GLI2 anticorps (Internal Region)

N° du produit ABIN129624

N° du produit (Fournisseur): 600-401-695

Cet anticorps anti-GLI2 est un anticorps Lapin Polyclonal détectant GLI2 dans WB, ELISA et IHC. Adapté pour Souris. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour GLI2 anticorps (Internal Region) (ABIN129624)

Antigène: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GLI2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-GLI2 anticorps

Épitope: Internal Region

N° du produit (Fournisseur): 600-401-695

Fournisseur: Rockland

Fonction: Gli2 Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against mouse Gli-2 protein.

Attributs du produit: Synonyms: rabbit anti-Gli-2 antibody, Gli 2, Gli2, zinc finger protein GLI2, Tax helper protein antibody, Thp antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids from an internal region of Mouse Gli-2.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 2 μg/mL to 20 μg/mL

Application Note: This antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. See figure legend for expectations by WB and IHC.  Multiple splice variants have been reported for this protein.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:15,000 - 1:60,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.02 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-GLI2 (ABIN129624)

Guntur, Reinhold, Cuellar, Naski: "Conditional ablation of Pten in osteoprogenitors stimulates FGF signaling." dans: Development (Cambridge, England), Vol. 138, Issue 7, pp. 1433-44, (2011) (PubMed).

Détails sur GLI2

Antigène: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Autre désignation: Gli2

Sujet: Background: Gli-2 (also known as Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein) belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling, and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli-2 is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes: Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, pre-axial polydactyly type IV, and postaxial polydactyly types A1 and B. Anti-Gli 2 Antibody is useful for researchers interested in transcription factor activities, DNA binding, and chromatin binding research.

ID gène: 14633, 124487481

UniProt: Q8K0K3

Pathways: Signalisation Hedgehog, Dopaminergic Neurogenesis