CBLC anticorps (C-Term)

N° du produit ABIN129659

N° du produit (Fournisseur): 600-401-889

L’anticorps Lapin Polyclonal anti-CBLC a été validé pour WB, ELISA, IHC et IP. Il convient pour détecter CBLC dans des échantillons de Humain.

Aperçu rapide pour CBLC anticorps (C-Term) (ABIN129659)

Antigène: CBLC (Cbl proto-oncogene C (CBLC))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CBLC est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Détail du produit anti-CBLC anticorps

Épitope: C-Term

N° du produit (Fournisseur): 600-401-889

Fournisseur: Rockland

Fonction: Cbl-c Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against human Cbl-c protein.

Attributs du produit: Synonyms: rabbit anti-Cbl-c Antibody, E3 ubiquitin-protein ligase CBL-C, RING finger protein 57, RING-type E3 ubiquitin transferase CBL-C, SH3-binding protein CBL-3, SH3-binding protein CBL-C, Signal transduction protein CBL-C, CBLC, CBL3, RNF57

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal portion of Human Cbl-c.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:500 - 1:3,000

Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, immunoprecipitation and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~52 kDa in size corresponding to Cbl-c by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:3,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.42 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur CBLC

Antigène: CBLC (Cbl proto-oncogene C (CBLC))

Autre désignation: CBLC

Sujet: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Cbl-c is also known as signal transduction protein CBL-C, SH3-binding protein CBL-C, CBL-3, and RING finger protein 57.  Cbl proteins are a family of ubiquitin protein ligases (E3s) that negatively regulate signaling by targeting activated tyrosine kinases for degradation.  Cbl-c (a.k.a. Cbl-3) is the most recently cloned member of the Cbl proteins and is expressed only in epithelial cells (the other Cbl proteins are ubiquitously expressed).  Cbl-c, like the other mammalian Cbl proteins, can ubiquitinate the activated EGFR and target it for degradation.  Cbl-c knock out mice show no obvious phenotype.  Thus, the physiological role of Cbl-c is not known.

ID gène: 23624, 125987803

UniProt: Q9ULV8

Pathways: EGFR Signaling Pathway