LIME anticorps (AA 86-202)

N° du produit ABIN1302445

Détail du produit anti-LIME anticorps

Antigène: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Épitope: AA 86-202

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LIME est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificité: Mouse monoclonal antibody mLIME-05 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed mainly by T cells.

Réactivité croisée (Details): Mouse, Other not tested

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Recombinant protein corresponding to amino acids 86-202 of mouse LIME

Clone: MLIME-05

Isotype: IgG2a

Information d'application

Indications d'application: Immunoprecipitation: Positive control: murine splenic T cells.
Western blotting: Reducing conditions, recommended dilution: 1-5 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Détails sur LIME

Antigène: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Autre désignation: LIME (LIME1 Produits)

Synonymes: anticorps LIME1, anticorps DKFZp459A158, anticorps LIME, anticorps LP8067, anticorps dJ583P15.4, anticorps 2810038K19Rik, anticorps RGD1562795, anticorps Lck interacting transmembrane adaptor 1, anticorps LIME1, anticorps Lime1

Sujet: Lck interacting transmembrane adaptor 1,LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.,Lita1

ID gène: 72699

UniProt: Q9EQR5