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LIME anticorps (AA 141-295)

LIME1 Reactivité: Humain IP, FACS Hôte: Souris Monoclonal LIME-06 unconjugated
N° du produit ABIN94424
  • Antigène Voir toutes LIME (LIME1) Anticorps
    LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))
    Épitope
    • 10
    • 9
    • 6
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 141-295
    Reactivité
    • 29
    • 3
    Humain
    Hôte
    • 22
    • 9
    • 1
    Souris
    Clonalité
    • 24
    • 8
    Monoclonal
    Conjugué
    • 16
    • 5
    • 3
    • 3
    • 3
    • 2
    Cet anticorp LIME est non-conjugé
    Application
    • 24
    • 16
    • 5
    • 3
    • 2
    • 1
    • 1
    Immunoprecipitation (IP), Flow Cytometry (FACS)
    Specificité
    The antibody LIME-06 was raised against intracellular fragment corresponding to aa 141-295 of human LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.
    Réactivité croisée (Details)
    Human
    Purification
    Purified by protein-A affinity chromatography.
    Pureté
    > 95 % (by SDS-PAGE)
    Immunogène
    Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME.
    Clone
    LIME-06
    Isotype
    IgG1
  • Indications d'application
    Flow cytometry: Recommended dilution: 8-12 μg/mL. Intracellular staining.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not freeze.
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8°C. Do not freeze.
  • Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." dans: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, (2003) (PubMed).

  • Antigène
    LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))
    Autre désignation
    LIME (LIME1 Produits)
    Synonymes
    anticorps LIME1, anticorps DKFZp459A158, anticorps LIME, anticorps LP8067, anticorps dJ583P15.4, anticorps 2810038K19Rik, anticorps RGD1562795, anticorps Lck interacting transmembrane adaptor 1, anticorps LIME1, anticorps Lime1
    Sujet
    Lck interacting transmembrane adaptor 1,LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.,LITA1
    ID gène
    54923
    UniProt
    Q9H400
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