CD40 Ligand anticorps

N° du produit ABIN1302520

Détail du produit anti-CD40 Ligand anticorps

Antigène: CD40 Ligand (CD40LG)

Reactivité: Souris

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp CD40 Ligand est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Functional Studies (Func)

Specificité: The Armenian hamster monoclonal antibody MR-1 detects an extracellular epitope on murine CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.

Réactivité croisée (Details): Mouse

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

niveau d'endotoxine: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogène: Activated mouse Th1 clone D1.6

Clone: MR-1

Isotype: IgG1

Information d'application

Indications d'application: Functional application:
Blocking T cell costimulation in vivo.
Flow cytometry: Recommended dilution: 4 μg/mL, positive control: 6-8 hour activated mouse splenocytes.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Agent conservateur: Azide free

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD40 Ligand (ABIN1302520)

Shin, Kennedy, Gorski, Tsuchiya, Koseki, Azuma, Yagita, Chen, Powell, Pardoll, Housseau: "Cooperative B7-1/2 (CD80/CD86) and B7-DC costimulation of CD4+ T cells independent of the PD-1 receptor." dans: The Journal of experimental medicine, Vol. 198, Issue 1, pp. 31-8, (2003) (PubMed).

Luzina, Atamas, Storrer, daSilva, Kelsoe, Papadimitriou, Handwerger: "Spontaneous formation of germinal centers in autoimmune mice." dans: Journal of leukocyte biology, Vol. 70, Issue 4, pp. 578-84, (2001) (PubMed).

Fischbein, Ardehali, Yun, Schoenberger, Laks, Irie, Dempsey, Cheng, Fishbein, Bonavida: "CD40 signaling replaces CD4+ lymphocytes and its blocking prevents chronic rejection of heart transplants." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 12, pp. 7316-22, (2000) (PubMed).

Hwang, Nahm, Briles, Thomas, Purkerson: "Acquired, but not innate, immune responses to Streptococcus pneumoniae are compromised by neutralization of CD40L." dans: Infection and immunity, Vol. 68, Issue 2, pp. 511-7, (2000) (PubMed).

Lode, Xiang, Pertl, Förster, Schoenberger, Gillies, Reisfeld: "Melanoma immunotherapy by targeted IL-2 depends on CD4(+) T-cell help mediated by CD40/CD40L interaction." dans: The Journal of clinical investigation, Vol. 105, Issue 11, pp. 1623-30, (2000) (PubMed).

Howard, Miga, Vanderlugt, Dal Canto, Laman, Noelle, Miller: "Mechanisms of immunotherapeutic intervention by anti-CD40L (CD154) antibody in an animal model of multiple sclerosis." dans: The Journal of clinical investigation, Vol. 103, Issue 2, pp. 281-90, (1999) (PubMed).

Salgado, Nakamura, Sugaya, Tada, Asahina, Koyama, Irie, Tamaki: "Functional CD40 ligand is expressed on epidermal Langerhans cells." dans: Journal of leukocyte biology, Vol. 66, Issue 2, pp. 281-5, (1999) (PubMed).

Noelle, Roy, Shepherd, Stamenkovic, Ledbetter, Aruffo: "A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 14, pp. 6550-4, (1992) (PubMed).

Détails sur CD40 Ligand

Antigène: CD40 Ligand (CD40LG)

Autre désignation: CD154 / CD40L (CD40LG Produits)

Synonymes: anticorps CD154, anticorps cd40l, anticorps CD40L, anticorps HIGM1, anticorps IGM, anticorps IMD3, anticorps T-BAM, anticorps TNFSF5, anticorps TRAP, anticorps gp39, anticorps hCD40L, anticorps CD40-L, anticorps Cd40l, anticorps Ly-62, anticorps Ly62, anticorps Tnfsf5, anticorps CD40 ligand, anticorps TNF superfamily member 5, anticorps CD40LG, anticorps cd40l, anticorps Cd40lg

Sujet: CD40 ligand,CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.,CD40L, CD40 ligand, TNFSF5, Ly62, gp39, T-BAM

ID gène: 959

UniProt: P29965

Pathways: Signalisation NF-kappaB, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints