DLG4 anticorps (AA 77-299)

N° du produit ABIN1304920

Cet anticorps Souris Monoclonal détecte spécifiquement DLG4 dans WB, IHC, ICC et IP. Il présente une réactivité envers Humain et a été mentionné dans 4+ publications.

Aperçu rapide pour DLG4 anticorps (AA 77-299) (ABIN1304920)

Antigène: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DLG4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: K28-43

Détail du produit anti-DLG4 anticorps

Épitope: AA 77-299

Specificité: No off-targets reported for recombinant Chapsyn-110, SAP97 or SAP102

Réactivité croisée: Poulet, Humain, Souris, Non-Human Primate, Rat

Attributs du produit:

Description: Our Anti-PSD 95 mouse monoclonal primary antibody is produced in-house from hybridoma clone K28/43. It is KO validated, detects chicken, human, mouse, non-human primate, and rat PSD 95, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Manufacturer Comment: We produce our PSD 95 mouse monoclonal primary antibody from hybridoma clone K28/43. It is great in IHC, ICC, IP, WB and is purified by Protein A chromatography.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography.

Pureté: > 90% specific antibody

Immunogène: Fusion protein amino acids 77-299 (PDZ domains 1 and 2) of human PSD-95 (accession number P78352) produced recombinantly in E. Coli

Isotype: IgG2a

Information d'application

Indications d'application:

Dilution Range: IP: 5 μg

Dilution Range: IHC: 1:500

Dilution Range: ICC: 1:500

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 10 mM Tris, 50 mM Sodium Chloride, 0.065 % Sodium Azide pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Date de péremption: 24 months

Références pour anticorps anti-DLG4 (ABIN1304920)

Madore, Leyrolle, Morel, Rossitto, Greenhalgh, Delpech, Martinat, Bosch-Bouju, Bourel, Rani, Lacabanne, Thomazeau, Hopperton, Beccari, Sere, Aubert, De Smedt-Peyrusse, Lecours, Bisht, Fourgeaud et al.: "Essential omega-3 fatty acids tune microglial phagocytosis of synaptic elements in the mouse developing brain. ..." dans: Nature communications, Vol. 11, Issue 1, pp. 6133, (2020) (PubMed).

Gonzalez-Lozano, Klemmer, Gebuis, Hassan, van Nierop, van Kesteren, Smit, Li: "Dynamics of the mouse brain cortical synaptic proteome during postnatal brain development." dans: Scientific reports, Vol. 6, pp. 35456, (2016) (PubMed).

Armstrong, Chung, Armstrong, Hochgeschwender, Jeong, Hawkes: "A novel somatostatin-immunoreactive mossy fiber pathway associated with HSP25-immunoreactive purkinje cell stripes in the mouse cerebellum." dans: The Journal of comparative neurology, Vol. 517, Issue 4, pp. 524-38, (2009) (PubMed).

Fischer, Foster, Scott, Sherwood: "Transient expression of LIM-domain transcription factors is coincident with delayed maturation of photoreceptors in the chicken retina." dans: The Journal of comparative neurology, Vol. 506, Issue 4, pp. 584-603, (2007) (PubMed).

Détails sur DLG4

Antigène: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Autre désignation: PSD 95

Sujet:

Synonyms: Disks large homolog 4 (Postsynaptic density protein 95) (PSD-95) (Synapse-associated protein 90) (SAP-90) (SAP90)

Target Description: PSD-95 is a member of the MAGUK (membrane associated guanylate kinase) family (which also includes PSD-93, SAP97 and SAP102) and functions as a scaffold protein that can cluster membrane receptors, ion channels, including NMDA receptor subunits, Kv1 potassium channels and Caspr2. The protein is encoded by DLG4, contains hallmark PDZ, SH3 and GuK domains and is found at post synaptic densities where it plays an important role in the formation and maintenance of synaptic junctions (Zhou and Blumberg, 2003). Indeed, overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. Antibodies against PSD95/DLG4 are frequently used in conjunction with pre-synaptic markers to localize synapses by microscopy

Gene Name Alternatives: DLG4 PSD95

Poids moléculaire: 95-110 kDa (varies with cell background due to phosphorylation)

UniProt: P78352

Pathways: Regulation of Muscle Cell Differentiation, Synaptic Membrane, Skeletal Muscle Fiber Development, Asymmetric Protein Localization, Regulation of long-term Neuronal Synaptic Plasticity