CD8 alpha anticorps (SPRD)

N° du produit ABIN135123

Cet anticorps anti-CD8 alpha est un anticorps Rat Monoclonal détectant CD8 alpha dans FACS. Adapté pour Souris.

Aperçu rapide pour CD8 alpha anticorps (SPRD) (ABIN135123)

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8 alpha est conjugé à/à la SPRD

Application: Flow Cytometry (FACS)

Clone: 53-6-7

Détail du produit anti-CD8 alpha anticorps (SPRD)

Marque: Spectral Red ™

Specificité: Mouse CD8α, Mr 32-34 kDa

Attributs du produit: Rat Anti-Mouse CD8a-SPRD

Immunogène: Spleen cells or thymocyte membranes

Isotype: IgG2a

Information d'application

Indications d'application:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Depletion - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 2 g/106 cells FITC and BIOT conjugates 2 g/106 cells AF488 conjugate 1 g/106 cells PE, APC, SPRD, PE/TXRD, CY5, PE/CY5.5, PE/CY7, 0.2 g/106 cells APC/CY5.5, APC/CY7, PACBLU, AF647, and AF700 conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Commentaires:

In vivo and in vitro depletion of CD8a+ cells

Volume d'échantillon: 1 mL

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C

Détails sur CD8 alpha

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Autre désignation: CD8a

Sujet: In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an chain (CD8/Lyt-2) and a chain (CD8/Lyt-3), and (ii) a homodimer of two chains. The heterodimer is found on the surface of essentially all thymocytes and the suppressor/cytotoxic subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8 without CD8. It has been suggested that CD8+- T cells mature extrathymically, while development of the CD8++ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8+ cells.

Pathways: TCR Signaling