GRP78 anticorps

N° du produit ABIN135301

Cet anticorps Rat Monoclonal détecte spécifiquement GRP78 dans WB. Il présente une réactivité envers Souris.

Aperçu rapide pour GRP78 anticorps (ABIN135301)

Antigène: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp GRP78 est non-conjugé

Application: Western Blotting (WB)

Clone: 76-E6

Détail du produit anti-GRP78 anticorps

Specificité: Mouse BiP, Mr 78 kDa

Attributs du produit: Rat Anti-Mouse BiP-UNLB

Purification: Purified

Immunogène: Mouse BiP: chain complexes

Isotype: IgG2a

Information d'application

Indications d'application:

• Applications: WB - Quality tested , IP - Reported in literature , ELISA - Reported in literature
• Working Dilutions: Immunoblotting Purified (UNLB) antibody 5 g/mL BIOT conjugate 5 g/mL

Volume d'échantillon: 1 mL

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Agent conservateur: Without preservative

Conseil sur la manipulation: Each reagent is stable for the period shown on the bottle label if stored as directed.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C

Détails sur GRP78

Antigène: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Autre désignation: BiP

Sujet: The immunoglobulin heavy chain binding protein BiP (Binding Protein) is a member of the hsp70 family of heat shock proteins, and is identical to the glucose regulated protein grp78.2 While BiP was originally described for its function in B cells, it is now known to be distributed in a variety of tissues, if not ubiquitous. The highly conserved hsp 70 proteins have an essential physiological role in stress responses and as molecular chaperones, which are responsible for a variety of functions such as protein transport, prevention of protein toxicity and direction of protein folding.1-5 With regard to its immunological role, BiP is a component of the endoplasmic reticulum and binds free intracellular heavy chains in nonsecreting pre-B cell lines (+,L-) or incompletely assembled Ig precursors in H+L+ secreting hybridomas and myelomas. In the absence of light chain synthesis, heavy chains remain associated with BiP and are not secreted. BiP is an ATP binding protein and the dissociation of the BiP-heavy chain complex is probably driven by the ATPase activity attributed to BiP.7

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling