H-2Kd/H-2Dd anticorps (Biotin)

N° du produit ABIN135418

Cet anticorps Souris Monoclonal détecte spécifiquement dans FACS. Il présente une réactivité envers Souris et a été mentionné dans 3+ publications.

Aperçu rapide pour H-2Kd/H-2Dd anticorps (Biotin) (ABIN135418)

Antigène: H-2Kd/H-2Dd

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Biotin

Application: Flow Cytometry (FACS)

Clone: 34-1-2S

Détail du produit

Specificité: Mouse H-2Kd/H-2Dd

Attributs du produit: Mouse Anti-Mouse H-2Kd/H-2Dd-BIOT

Immunogène: BDF1 mouse splenocytes

Isotype: IgG2a

Information d'application

Indications d'application:

• Applications: FC - Quality tested , ICC - Reported in literature , EM - Reported in literature , IP - Reported in literature , CMCD - Reported in literature , Block - Reported in literature , Adhesion - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Commentaires:

Blocking Cell Adhesion, Complement-mediated cytotoxicity

Volume d'échantillon: 1 mL

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C

Référence

Abel, Goto, Magnuson, Abraham, Ramanathan, Hotaling, Alaniz, Kumar-Sinha, Dziubinski, Urs, Wang, Shi, Waghray, Ljungman, Crawford, Simeone: "HNF1A is a novel oncogene that regulates human pancreatic cancer stem cell properties." dans: eLife, Vol. 7, (2019) (PubMed).

Pal, Dziubinski, Di Magliano, Simeone, Owens, Thomas, Peterson, Potu, Talpaz, Donato: "Usp9x Promotes Survival in Human Pancreatic Cancer and Its Inhibition Suppresses Pancreatic Ductal Adenocarcinoma In Vivo Tumor Growth." dans: Neoplasia (New York, N.Y.), Vol. 20, Issue 2, pp. 152-164, (2018) (PubMed).

Chen, Fisher, Signs, Molina, Shenoy, Lopez, Baker, Koomen, Chen, Gittleman, Barnholtz-Sloan, Berg, Appelman, Huang: "Inhibition of PI3K/Akt/mTOR signaling in PI3KR2-overexpressing colon cancer stem cells reduces tumor growth due to apoptosis." dans: Oncotarget, Vol. 8, Issue 31, pp. 50476-50488, (2017) (PubMed).

Détail du antigène

Antigène: H-2Kd/H-2Dd

Sujet: The classical MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic chains noncovalently associated with the invariant 2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.