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CD19 anticorps (AA 151-250)

Name: CD19 anticorps (AA 151-250)
SKU: ABIN1387313
Price: 414.62 EUR
Availability: InStock

CD19 Reactivité: Humain, Souris, Rat FACS, WB, ELISA, IHC (p) Hôte: Lapin Polyclonal unconjugated

Mouse splenocytes probed with Rabbit Anti-CD19 Polyclonal Antibody, PE-Cy5 Conjugated .

FACS

Antigen: 2 µg/100 µL Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Rabbit Anti-Goat IgG at 1: 5000; TMB staining Read the data in Microplate Reader by 450nm.

ELISA

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-CD19 Polyclonal Antibody, Unconjugated (ABIN1387313) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

IHC

Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-CD19 Polyclonal Antibody, Unconjugatedused at 1:200 dilution for 40 minutes at 37°C.

IF (p)

Mouse lung and liver lysates probed with CD19 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Paraformaldehyde-fixed, paraffin embedded fetal mouse brain tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD19 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse spleen tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD19 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining

IHC (p)

7 images

N° du produit ABIN1387313

Fiche de données

Antigène Voir toutes CD19 Anticorps

CD19 (CD19 Molecule (CD19))
Épitope
28

19

16

15

10

7

5

4

4

4

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 151-250
Reactivité
303

179

47

30

19

10

7

6

3

3

2

2

2

2

2

2

2

2
Humain, Souris, Rat
Hôte
185

139

63

4
Lapin
Anticorps secondaires appropriés
Clonalité
260

127

2
Polyclonal
Conjugué
116

25

22

21

18

9

9

9

8

8

8

7

7

7

7

7

6

6

5

5

4

4

4

4

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1
Cet anticorp CD19 est non-conjugé
Application
273

119

74

66

61

42

22

21

17

14

14

10

10

5

4

4

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Réactivité croisée

Humain, Souris, Rat

Homologie

Pig,Guinea Pig

Purification

Purified by Protein A.

Immunogène

KLH conjugated synthetic peptide derived from human CD19

Isotype

IgG

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CD19 Reactivité: Humain FACS, WB, IF, ICC, IHC (p) Hôte: Lapin Polyclonal unconjugated

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CD19 Reactivité: Humain FACS, WB, IF, IHC, ELISA Hôte: Lapin Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 160-258) antibody
CD19 Reactivité: Humain WB, IP, IHC, ICC Hôte: Lapin Polyclonal unconjugated

anti-CD19 Molecule (CD19) antibody
CD19 Reactivité: Humain FACS, IP Hôte: Souris Monoclonal LT19 unconjugated

anti-CD19 Molecule (CD19) antibody
CD19 Reactivité: Humain FACS, ICC Hôte: Souris Monoclonal 4G7 unconjugated

Indications d'application

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur

ProClin

Précaution d'utilisation

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption

12 months
Antigène

CD19 (CD19 Molecule (CD19))

Autre désignation

CD19 (CD19 Produits)

Synonymes

anticorps B4, anticorps CVID3, anticorps AW495831, anticorps CD19, anticorps CD19 molecule, anticorps CD19 antigen, anticorps CD19, anticorps Cd19

Sujet

Synonyms: B4, CVID3, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12, CD19
Background: Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

ID gène

930

UniProt

P15391

Pathways

Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway