RIT1 anticorps (AA 141-219) (HRP)

N° du produit ABIN1416694

Cet anticorps Lapin Polyclonal détecte spécifiquement RIT1 dans WB, ELISA, IHC (p) et IHC (fro). Il présente une réactivité envers Humain.

Aperçu rapide pour RIT1 anticorps (AA 141-219) (HRP) (ABIN1416694)

Antigène: RIT1 (Ras-Like Without CAAX 1 (RIT1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RIT1 est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-RIT1 anticorps (HRP)

Épitope: AA 141-219

Homologie: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human RIT1

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur RIT1

Antigène: RIT1 (Ras-Like Without CAAX 1 (RIT1))

Autre désignation: RIT1

Sujet:

Synonyms: GTP binding protein Roc1, GTP-binding protein Rit1, Ras like protein expressed in many tissues, Ras like without CAAX 1, Ras-like protein expressed in many tissues, Ras-like without CAAX protein 1, RIBB, Ric like expressed in many tissues, RIT, RIT1, RIT1_HUMAN, ROC1.

Background: Plays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation.Neuronal activity dramatically increases the concentration of cytosolic Ca2+, which then serves as a second messenger to direct diverse cellular responses. Calmodulin is a primary mediator of Ca2+ signals in the nervous system. Ric, a protein related to the Ras subfamily of small GTPases, has the ability to bind calmodulin. In addition, two Ras-like human proteins, Rin and Rit (Ric-related gene expressed in many tissues), which are 71 % and 66 % identical to RIC respectively, share related G2 domains with Ric. While most members of the Ras subfamily are plasma membrane-associated and generally require a C-terminal isoprenyl group to bind to the plasma membrane, Rit and Rin lack the recognition signal for C-terminal prenylation. Transiently expressed Rit and Rin are plasma membrane-localized because both proteins contain a C-terminal cluster of basic amino acids, which provides a mechanism for membrane association. Rin binds calmodulin through a C-terminal binding motif. Rit and Ric are widely expressed, whereas expression of Rin is restricted to the neuron system. In conclusion, Rit and Rin define a novel subfamily of Ras-related proteins

ID gène: 6016

Pathways: Neurotrophin Signaling Pathway