ATG4A anticorps (C-Term)

N° du produit ABIN1449633

Cet anticorps anti-ATG4A Polyclonal Lapin (ABIN1449633) détecte spécifiquement ATG4A dans WB, IHC (p) et EIA. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour ATG4A anticorps (C-Term) (ABIN1449633)

Antigène: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATG4A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-ATG4A anticorps

Épitope: AA 369-398, C-Term

Purification: Saturated Ammonium Sulfate precipitation followed by dialysis against PBS

Immunogène: KLH conjugated synthetic peptide between 369~398 amino acids from the C-term of Human APG4A

Isotype: Ig Fraction

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur ATG4A

Antigène: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Autre désignation: Autophagin-2

Sujet: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4A is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.Synonyms: APG4A, ATG4A, AUT-like 2 cysteine endopeptidase, AUTL2, Autophagy-related cysteine endopeptidase 2, Autophagy-related protein 4 homolog A, Cysteine protease ATG4A

Poids moléculaire: 45378 Da

ID gène: 115201

NCBI Accession: NP_443168

Pathways: Autophagy