AMPK alpha anticorps (pThr172)

N° du produit ABIN1531172

Cet anticorps anti-AMPK alpha Polyclonal Lapin (ABIN1531172) détecte spécifiquement AMPK alpha dans WB, IHC, ELISA et IF. L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Singe, Porc et Shrimp.

Aperçu rapide pour AMPK alpha anticorps (pThr172) (ABIN1531172)

Antigène: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Reactivité: Humain, Souris, Rat, Singe, Porc, Shrimp

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AMPK alpha est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Détail du produit anti-AMPK alpha anticorps

Épitope: AA 140-189, pThr172

Fonction: AMPKα1/2 (phospho Thr183/172) Antibody

Specificité: Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody detects endogenous levels of AMPKα1/2 protein only when phosphorylated at T183/172.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pureté: > 95 %

Immunogène: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Isotype: IgG

Information d'application

Indications d'application: IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000. Not yet tested in other applications.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C/1 year

Date de péremption: 12 months

Détails sur AMPK alpha

Antigène: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Autre désignation: AMPK alpha

Sujet:

Synonyms: PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK;

Background: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],

Gene Name: AAPK1/AAPK2

Protein Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1/2

Poids moléculaire: 62 kDa

ID gène: 5562, 5563

OMIM: 602739

UniProt: Q13131

Pathways: L'effet Warburg