Prostate Specific Antigen anticorps

N° du produit ABIN1543584

Cet anticorps anti-Prostate Specific Antigen est un anticorps Souris Monoclonal détectant Prostate Specific Antigen dans ELISA, WB, FACS et DB. Adapté pour Humain. Validé indépendamment pour une utilisation dans Flow Cytometry.

Aperçu rapide pour Prostate Specific Antigen anticorps (ABIN1543584)

Antigène: Prostate Specific Antigen (PSA)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Prostate Specific Antigen est non-conjugé

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Dot Blot (DB)

Clone: A5D5-1

Détail du produit anti-Prostate Specific Antigen anticorps

Purification: Protein A affinity column

Immunogène: Native PSA Antigen

Isotype: IgG1

Information d'application

Commentaires:

Other Applications have not been tested.

Restrictions: For Research Use only

Independent Validation (FACS)

Validation #029617 (Flow Cytometry)

by: Flow Cytometry & Cell Separation Facility, Purdue University

No.: #029617

Date: 22.02.2014

Numéro du lot: FAPS-A5D5-0003

Application validée: Flow Cytometry

Contrôle positif: LNCaP cells

Contrôle négative: - DU145 cells - Jurkat cells

Conclusion: Strong signal above isotype signal was observed in the positive control sample, while staining of similar level to isotype control was observed in both negative control samples.

Validation Images

Figure 1: LNCaP cells stained with anti-PSA (blue) or with isotype control (red).

Figure 2: DU145 cells stained with anti-PSA (blue) or with isotype control (red).

Figure 3: Jurkat cells stained with anti-PSA (blue) or with isotype control (red).

Protocole

Anticorps primaire:

• Antibody: Prostate Specific Antigen (PSA)
• Catalog number: ABIN1543584
• Lot number: FAPS-A5D5-0003

Anticorps secondaire:

• Antibody: F(ab’)2 goat anti-mouse IgG-Alexa 647

Full Protocol:

• Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with 0.05% trypsin / 0.025% EDTA.
• Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
• An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37°C.
• Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
• Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA and 0.1% Tween 20
• Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA.
• 1 µg of anti-PSA or mouse IgG1 isotype control were added to the cells and incubated for 30 min at room temperature
• Cells were washed twice with PBS/BSA/Tween 20
• Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA
• Secondary antibody was added at a final dilution of 1:500 and incubated for 30 min at room temperature
• Cells were washed twice with PBS/BSA/Tween 20
• Cells were resuspended in 0.3 mL PBS/BSA
• Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).

Notes: None

Stockage

Format: Liquid

Concentration: 1.8 mg/mL

Buffer: PBS, pH 7.4, 0.09% sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Détails sur Prostate Specific Antigen

Antigène: Prostate Specific Antigen (PSA)

Autre désignation: PSA

Pathways: Système du Complément