Podoplanin anticorps (Biotin)

N° du produit ABIN155160

Cet anticorps anti-Podoplanin est un anticorps Souris Monoclonal détectant Podoplanin dans FACS. Adapté pour Humain. Ce Primary Antibody a été cité dans 3+ publications.

Aperçu rapide pour Podoplanin anticorps (Biotin) (ABIN155160)

Antigène: Podoplanin (PDPN)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Podoplanin est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Clone: 18H5

Détail du produit anti-Podoplanin anticorps (Biotin)

Specificité: This antibody reacts with Podoplanin (gp36). The unconjugated monoclonal antibody will detect native Human Podoplanin in Western Blot and on the surface of Human LEC.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G

Immunogène: Podoplanin-expressing MDCK cells.

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: use at 2-5 μg/mL together with the appropriate secondary reagents.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL. Centrifuge vial prior to opening.

Buffer: PBS, 0.02 % Sodium Azide, BSA (50x)

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Références pour anticorps anti-Podoplanin (Biotin) (ABIN155160)

Conrad, Niess, Huss, Huber, von Luettichau, Nelson, Ott, Jauch, Bruns: "Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo." dans: Circulation, Vol. 119, Issue 2, pp. 281-9, (2009) (PubMed).

Turner, Langley, Johnston, Gentil, Ford, Wu, Graham, Sharpley, Slatko, Pearlman, Taylor: "Wolbachia lipoprotein stimulates innate and adaptive immunity through Toll-like receptors 2 and 6 to induce disease manifestations of filariasis." dans: The Journal of biological chemistry, Vol. 284, Issue 33, pp. 22364-78, (2009) (PubMed).

Kilic, Oliveira-Ferrer, Neshat-Vahid, Irmak, Obst-Pernberg, Wurmbach, Loges, Kilic, Weil, Lauke, Tilki, Singer, Ergün: "Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1." dans: Blood, Vol. 110, Issue 13, pp. 4223-33, (2007) (PubMed).

Détails sur Podoplanin

Antigène: Podoplanin (PDPN)

Autre désignation: Podoplanin

Sujet: Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential Olinked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47 % aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for Ctype lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on Oglycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.Synonyms: Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha

ID gène: 10630

NCBI Accession: NP_001006625

UniProt: Q86YL7

Pathways: Dicarboxylic Acid Transport