Podoplanin anticorps

N° du produit ABIN155169

Cet anticorps anti-Podoplanin est un anticorps Hamster Monoclonal détectant Podoplanin dans WB, IP, IHC (p) et IHC (fro). Adapté pour Souris. Ce Primary Antibody a été cité dans 3+ publications.

Aperçu rapide pour Podoplanin anticorps (ABIN155169)

Antigène: Podoplanin (PDPN)

Reactivité: Souris

Hôte: Hamster

Clonalité: Monoclonal

Conjugué: Cet anticorp Podoplanin est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: RTD4E10

Détail du produit anti-Podoplanin anticorps

Specificité: The monoclonal antibody will detect Podoplanin on the surface of lymphatic endothelial cells and some epithelial cell types by immunostaining or immunohistochemistry. The antibody does not cross-react with Human Podoplanin.

Purification: Protein G Chromatography

Immunogène: Murine thymic stromal cell line

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry (use diluted 1-10 μg/mL IgG). Works well with paraffin sections. Western blot (use 1-10 μg/mL IgG, will detect a 36-40 kDa band). Immunoprecipitation (use 1-5 μg IgG per 1 mLlysate or reaction volume).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.4, without preservatives or stabilizers

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. After reconstitution store the antibody (in aliquots) at -20 °C.

Références pour anticorps anti-Podoplanin (ABIN155169)

Uhrin, Zaujec, Breuss, Olcaydu, Chrenek, Stockinger, Fuertbauer, Moser, Haiko, Fässler, Alitalo, Binder, Kerjaschki: "Novel function for blood platelets and podoplanin in developmental separation of blood and lymphatic circulation." dans: Blood, Vol. 115, Issue 19, pp. 3997-4005, (2010) (PubMed).

DAmico, Jones, Nye, Sapienza, Ramjuan, Reynolds, Robinson, Kostourou, Martinez, Aubyn, Grose, Thomas, Spencer-Dene, Zicha, Davies, Tybulewicz, Hodivala-Dilke: "Regulation of lymphatic-blood vessel separation by endothelial Rac1." dans: Development (Cambridge, England), Vol. 136, Issue 23, pp. 4043-53, (2009) (PubMed).

NOYES, DOMM, WILLSLC: "Regulation of erythropoiesis. I. Erythropoietin assay as a clinical tool." dans: Blood, Vol. 20, pp. 9-18 (PubMed).

Détails sur Podoplanin

Antigène: Podoplanin (PDPN)

Autre désignation: Podoplanin

Sujet: Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a Mr 38,000 membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.Synonyms: Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha

ID gène: 14726

NCBI Accession: NP_034459

UniProt: Q62011

Pathways: Dicarboxylic Acid Transport