TIE1 anticorps (Biotin)

N° du produit ABIN155170

Cet anticorps Souris Monoclonal détecte spécifiquement TIE1 dans WB, FACS et EIA. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour TIE1 anticorps (Biotin) (ABIN155170)

Antigène: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TIE1 est conjugé à/à la Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Clone: 6F12

Détail du produit anti-TIE1 anticorps (Biotin)

Specificité: This unconjugated monoclonal antibody will detect native TIE-1 in ELISA experiments and on the surface of different cell types.

Purification: Protein G Chromatography.

Immunogène: Recombinant human soluble TIE-1 protein.

Isotype: IgG1

Information d'application

Indications d'application: ELISA: 1-15 μg/mL. Western blot: 1-2 μg/mL. FACS: 2-5 μg/mL. Cell sorting: 2-5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL

Buffer: PBS, 0.02 % Sodium Azide, BSA (50X)

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: This product is photosensitive and should be protected from light

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted Prior to and After reconstitution at 2-8 °C. DO NOT FREEZE!

Détails sur TIE1

Antigène: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Autre désignation: TIE1

Sujet: The soluble receptor protein consists of the full extracellular domain (Met1-Glu749). The recombinant mature TIE-1/Fc is a disulfide-linked homodimeric protein. Human TIE-1/Fc monomer has a calculated molecular mass of approximately 105 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 125 kDa protein in SDS-PAGE under reducing conditions.TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human TIE-1 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Whereas two ligands have been described for TIE-2 [angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2)], so far no ligand was found for TIE-1.Synonyms: TIE, Tie-1, Tyrosine-protein kinase receptor Tie-1

ID gène: 7075

NCBI Accession: NP_005415

UniProt: P35590

Pathways: Signalisation RTK