TIE1 anticorps

N° du produit ABIN155171

L’anticorps anti-TIE1 Monoclonal Souris est utilisé pour la détection de TIE1 dans des échantillons de Humain. Il a été validé pour WB, FACS et EIA.

Aperçu rapide pour TIE1 anticorps (ABIN155171)

Antigène: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TIE1 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Clone: 6F12

Détail du produit anti-TIE1 anticorps

Specificité: This antibody will detect native TIE-1 in ELISA experiments and on the surface of different cell types.

Purification: Protein G chromatography

Immunogène: Recombinant human soluble TIE-1 protein

Isotype: IgG1

Information d'application

Indications d'application: ELISA: 1-15 μg/mL. Western blot: 1-2 μg/mL. FACS. Cell sorting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS, pH 7.4 without preservatives or stabilizers

Agent conservateur: Without preservative

Conseil sur la manipulation: This product is photosensitive and should be protected from light

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted Prior to and After reconstitution at 2-8 °C. DO NOT FREEZE!

Détails sur TIE1

Antigène: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Autre désignation: TIE1

Sujet: TIE1/TIE (tyrosine kinase with Ig and EGF homology domains 1) and TIE2/Tek define a new class of the receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin like domains flanking three epidermal growth factor (EFG) like domains followed by three fibronectin type III like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. Human TIE1 cDNA encodes a 1138 amino acid residue precursor protein with a putative signal peptide, an extracellular domain, and a cytoplasmic domain. Human TIE1/Fc, a disulfide linked homodimeric protein, has a calculated molecular mass of approximately 107 kDa. Due to glycosylation, the protein migrates to approximately 160 kDa in SDS PAGE under reducing conditions. TIE1 and TIE2, expressed primarily on endothelial and hematopoietic progenitor cells, play important roles in angiogenesis, vasculogenesis, and hematopoiesis. In developing vascular endothelial cells, TIE1 and TIE2 are specifically expressed. Two ligands that bind TIE have been identified, angiopoietin 1 and angiopoietin 2. Based on gene targeting studies, the in vivo functions of TIE1 are related to endothelial cell differentiation. The receptor tyrosine kinase TIE also plays a role in the survival and integrity of the endothelium.Synonyms: TIE, Tie-1, Tyrosine-protein kinase receptor Tie-1

ID gène: 7075

NCBI Accession: NP_005415

UniProt: P35590

Pathways: Signalisation RTK