HSP27 anticorps

N° du produit ABIN1580426

L’anticorps anti-HSP27 Monoclonal Souris est utilisé pour la détection de HSP27 dans des échantillons de Humain, Boeuf (Vache), Souris, Porc, Rat et Mammifères. Il a été validé pour WB, IHC, ICC et IF.

Aperçu rapide pour HSP27 anticorps (ABIN1580426)

Antigène: HSP27 (Heat Shock Protein 27 (HSP27))

Reactivité: Humain, Boeuf (Vache), Souris, Porc, Rat, Mammifères

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HSP27 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 6H11

Détail du produit anti-HSP27 anticorps

Purification: affinity purified antibody

Isotype: IgG1

Information d'application

Indications d'application: The antibody solution can be used at dilutions of at least 1:1,000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10,000 or lower.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 4°C short term or -20°C long term.

Détails sur HSP27

Antigène: HSP27 (Heat Shock Protein 27 (HSP27))

Autre désignation: Heat shock protein 27- HSP27

Sujet: The heat shock proteins were discovered, as the name suggests, since they are heavily upregulated when cells are stressed by temperatures above the normal physiological range. They are expressed in unstressed cells also and have a normal function as chaperones, helping other proteins to fold correctly, and are required in much greater amounts if the cell or tissue is stressed by heat. The increased levels are generated transcriptionally under the influence of a powerful transcription factor, the heat shock factor 1 (HSF1). The different heat shock proteins were originally named based on their SDS-PAGE mobility, so HSP27 has an apparent molecular weight of 27 kDa. It is an abundant protein even under non-stress conditions and frequently shows up as a major spot on 2 dimensional gels of cells or tissues. It is known to associate with a variety of other proteins such as actin, intermediate filament subunits and ubiquitin and is found both in the cytoplasm and the nucleus of cells. HSP27 can become heavily phosphorylated under the influence of multiple protein kinases particularly as a result of activation of the p38/SAPK pathway. Upregulation of this protein is protective against neurodegenerative diseases at least in certain mouse models. Point mutations in the HSP27 gene are associated with two neurological diseases, Charcot-Marie-Tooth disease type 2F and distal hereditary motor neuropathy IIB. These diseases are associated with axonal loss apparently following defects in the transport of neurofilaments. The HGNC name for this protein is HSBP1.

Pathways: VEGF Signaling