FLT1 anticorps (Biotin)

N° du produit ABIN1589706

Cet anticorps anti-FLT1 est un anticorps Souris Monoclonal détectant FLT1 dans WB, IHC, ELISA et FACS. Adapté pour Humain.

Aperçu rapide pour FLT1 anticorps (Biotin) (ABIN1589706)

Antigène: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FLT1 est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Clone: EWC

Détail du produit anti-FLT1 anticorps (Biotin)

Specificité: Recombinant human soluble extracellular Flt-1 (D5)

Réactivité croisée: Souris

Attributs du produit: Chromosomal location: 13q12
The monoclonal antibody was produced with the help of BALB/c mice using recombinant human soluble extracellular Flt-1 (D5) as the immunizing antigen. The monoclonal antibody will detect native and denatured human VEGFR-1/Flt-1 in ELISA experiments and on the surface of different human cell types. The antibody will also detect mouse Flt-1 (e.g. natural occurring soluble mouse Flt-1).

Purification: Mouse IgG1 antibody (clone EWC) from hybridomas was purified from cell culture supernatant by Protein G chromatography.

Isotype: IgG1

Information d'application

Indications d'application: ELISA: use at 1–10 µg/mL, Western blotting: use at 2–5 µg/mL, FC: use at 2-10 µg/mL

Commentaires:

Monoclonal Antibody

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, BSA (50x), 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Centrifuge vial prior to opening.

Stock: -20 °C

Stockage commentaire: The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C.

Date de péremption: 6 months

Détails sur FLT1

Antigène: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Autre désignation: VEGFR-1/Flt-1

Sujet: Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVE supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA.
Synonyms: vascular endothelial growth factor receptor-1, FLT1, VEGF receptor 1,

NCBI Accession: NM_001159920, NP_001153392

UniProt: P17948

Pathways: Signalisation RTK, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals