Podoplanin anticorps

N° du produit ABIN1589801

L’anticorps Souris Monoclonal anti-Podoplanin a été validé pour WB, IHC, FACS et IF. Il convient pour détecter Podoplanin dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour Podoplanin anticorps (ABIN1589801)

Antigène: Podoplanin (PDPN)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Podoplanin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 18H5

Détail du produit anti-Podoplanin anticorps

Specificité: Gp36 (Podoplanin)-expressing MDCK cells

Attributs du produit: Chromosomal location: 1p36.21
The monoclonal antibody was produced with the help of BALB/c mice using MDCK cells stably expressing gp36 (Podoplanin) protein as the immunizing antigen.

Purification: The mouse IgG1 antibody (#18H5) from hybridomas was purified from cell culture supernatant by Protein G chromatography.

Isotype: IgG1

Information d'application

Indications d'application: Western Analysis: use at 1-5 µg/mL, Immunofluorescence: use at 6-30 µg/mL, FACS analysis: use at 1-10 µg/mL, Immunohistochemistry: use at 6-30 µg/mL.

Commentaires:

Monoclonal Antibody

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of > 0.5 mg/mL

Buffer: PBS

Conseil sur la manipulation: Centrifuge vial prior to opening.

Stock: -20 °C

Stockage commentaire: The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C.

Date de péremption: 6 months

Références pour anticorps anti-Podoplanin (ABIN1589801)

Steiniger, Wilhelmi, Seiler, Lampp, Stachniss: "Heterogeneity of stromal cells in the human splenic white pulp. Fibroblastic reticulum cells, follicular dendritic cells and a third superficial stromal cell type." dans: Immunology, Vol. 143, Issue 3, pp. 462-77, (2014) (PubMed).

Détails sur Podoplanin

Antigène: Podoplanin (PDPN)

Autre désignation: Podoplanin

Sujet: Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential O-linked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on O-glycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.
Synonyms: PDPN, T1A, GP36, GP40, Gp38, OTS8, T1A-2, AGGRUS, HT1A-1, PA2.26

NCBI Accession: NM_001006624, NP_001006625

UniProt: Q86YL7

Pathways: Dicarboxylic Acid Transport