Lactate Dehydrogenase A anticorps (Biotin)

N° du produit ABIN1607671

N° du produit (Fournisseur): 200-1673-0100

L’anticorps Chèvre Polyclonal anti-Lactate Dehydrogenase A a été validé pour WB, ELISA, IP et IF. Il convient pour détecter Lactate Dehydrogenase A dans des échantillons de Lapin. Il y a 1 publication disponible.

Aperçu rapide pour Lactate Dehydrogenase A anticorps (Biotin) (ABIN1607671)

Antigène: Lactate Dehydrogenase A (LDHA)

Reactivité: Lapin

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Lactate Dehydrogenase A est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Détail du produit anti-Lactate Dehydrogenase A anticorps (Biotin)

N° du produit (Fournisseur): 200-1673-0100

Fournisseur: Rockland

Fonction: Lactate Dehydrogenase Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].

Attributs du produit: Synonyms: goat anti-Lactate Dehydrogenase Antibody biotin Conjugation, biotin Conjugated goat anti-Lactate Dehydrogenase Antibody, L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit, LDH-M

Purification: Anti-lactate dehydrogenase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Lactate Dehydrogenase [full length protein isolated from rabbit muscle]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Lactate Dehydrogenase Biotin antibody has been tested by ELISA, Western blot, and immunofluorescence. This antibody is assayed against 1.0 μg of Lactate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:2,500 - 1:6,500

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Références pour anticorps anti-Lactate Dehydrogenase A (Biotin) (ABIN1607671)

Vincent, Cornea, Jong, Laferrière, Kumar, Mickeviciute, Fung, Bandegi, Ribeiro-da-Silva, OMalley, Coderre: "Intracellular mGluR5 plays a critical role in neuropathic pain." dans: Nature communications, Vol. 7, pp. 10604, (2016) (PubMed).

Détails sur Lactate Dehydrogenase A

Antigène: Lactate Dehydrogenase A (LDHA)

Autre désignation: LDHA

Sujet: Background: Anti-lactate dehydrogenase recognizes lactate dehydrogenase which catalyzes the interconversion of pyruvate to lactate and NADH to NAD+. This reaction occurs in the absence or lack of oxygen, making lactate dehydrogenase critical for anaerobic respiration. There five known isozymes of LDH, each localized in different types of tissue. In medicine, LDH is often used as a marker of various diseases ranging from hemolysis to encephalitis. Anti-lactate dehydrogenase antibody is ideal for investigators interested in Metabolism, Cancer, and Signal Transduction.

ID gène: 100009107, 126050

UniProt: P13491

Pathways: L'effet Warburg